Structure and Metabolically Oriented Efficacy of Fucoidan from Brown Alga Sargassum muticum in the Model of Colony Formation of Melanoma and Breast Cancer Cells

• Published in: Marine drugs Vol. 21; no. 9; p. 486
• Main Authors: Usoltseva, Roza V, Zueva, Anastasiya O, Malyarenko, Olesya S, Anastyuk, Stanislav D, Moiseenko, Olga P, Isakov, Vladimir V, Kusaykin, Mikhail I, Jia, Airong, Ermakova, Svetlana P
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.09.2023; MDPI
• Subjects: 2-deoxy- d -glucose; Acids; Algae; Breast cancer; Cancer; Cancer therapies; Cells; Colonies; Cytotoxicity; Disaccharides; Fucoidan; Fucose; Galactose; Glucose; Glycolysis; Humans; Inhibitors; Investigations; Kinases; Mass spectrometry; MDA-MB-231; Melanoma; Metabolism; Polysaccharides - pharmacology; Residues; Sargassum; Sargassum muticum; Scientific imaging; SK-MEL-28 cells; Sargassum muticum; SK-MEL-28 cells; 2-deoxy-d-glucose; glycolysis; anticancer activity; fucoidan; MDA-MB-231
• ISSN: 1660-3397; 1660-3397
• DOI: 10.3390/md21090486

This work reports the detailed structure of fucoidan from (2SmF2) and its ability to potentiate the inhibitory effect of glycolysis inhibitor 2-deoxy-d-glucose (2-DG). 2SmF2 was shown to be sulfated and acetylated galactofucan containing a main chain of alternating residues of 1,3- and 1,4-linked α-l-fucopyranose, fucose fragments with monotonous 1,3- and 1,4-type linkages (DP up to 3), α-d-Gal-(1→3)-α-L-Fuc disaccharides, and 1,3,4- and 1,2,4-linked fucose branching points. The sulfate groups were found at positions 2 and 4 of fucose and galactose residues. 2SmF2 (up to 800 µg/mL) and 2-DG (up to 8 mM) were not cytotoxic against MDA-MB-231 and SK-MEL-28 as determined by MTS assay. In the soft agar-based model of cancer cell colony formation, fucoidan exhibited weak inhibitory activity at the concentration of 400 µg/mL. However, in combination with low non-cytotoxic concentrations of 2-DG (0.5 or 2 mM), 2SmF2 could effectively inhibit the colony formation of SK-MEL-28 and MDA-MB-231 cells and decreased the number of colonies by more than 50% compared to control at the concentration of 200 µg/mL. Our findings reveal the metabolically oriented effect of fucoidan in combination with a glycolysis inhibitor that may be beneficial for a therapy for aggressive cancers.