The Synergistic Priming Effect of Exogenous Salicylic Acid and H2O2 on Chilling Tolerance Enhancement during Maize (Zea mays L.) Seed Germination
Chilling stress is an important constraint for maize seedling establishment in the field. To examine the role of salicylic acid (SA) and hydrogen peroxide (H 2 O 2 ) in response to chilling stress, we investigated the effects of seed priming with SA, H 2 O 2 , and SA+H 2 O 2 combination on maize res...
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| Published in | Frontiers in plant science Vol. 8; p. 1153 |
|---|---|
| Main Authors | , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Frontiers Media S.A
05.07.2017
|
| Subjects | |
| Online Access | Get full text |
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| Summary: | Chilling stress is an important constraint for maize seedling establishment in the field. To examine the role of salicylic acid (SA) and hydrogen peroxide (H
2
O
2
) in response to chilling stress, we investigated the effects of seed priming with SA, H
2
O
2
, and SA+H
2
O
2
combination on maize resistance under chilling stress (13°C). Priming with SA, H
2
O
2
, and especially SA+H
2
O
2
shortened seed germination time and enhanced seed vigor and seedling growth as compared with hydropriming and non-priming treatments under low temperature. Meanwhile, SA+H
2
O
2
priming notably increased the endogenous H
2
O
2
and SA content, antioxidant enzymes activities and their corresponding genes
ZmPAL, ZmSOD4, ZmAPX2, ZmCAT2
, and
ZmGR
expression levels. The α-amylase activity was enhanced to mobilize starch to supply metabolites such as soluble sugar and energy for seed germination under chilling stress. In addition, the SA+H
2
O
2
combination positively up-regulated expressions of gibberellic acid (GA) biosynthesis genes
ZmGA20ox1
and
ZmGA3ox2
, and down-regulated GA catabolism gene
ZmGA2ox1
expression; while it promoted GA signaling transduction genes expressions of
ZmGID1
and
ZmGID2
and decreased the level of seed germination inhibitor gene
ZmRGL2
. The abscisic acid (ABA) catabolism gene
ZmCYP707A2
and the expressions of
ZmCPK11
and
ZmSnRK2.1
encoding response receptors in ABA signaling pathway were all up-regulated. These results strongly suggested that priming with SA and H
2
O
2
synergistically promoted hormones metabolism and signal transduction, and enhanced energy supply and antioxidant enzymes activities under chilling stress, which were closely relevant with chilling injury alleviation and chilling-tolerance improvement in maize seed.
Highlights:
Seed germination and seedling growth were significantly improved under chilling stress by priming with SA+H
2
O
2
combination, which was closely relevant with the change of reactive oxygen species, metabolites and energy supply, hormones metabolism and regulation. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Plant Physiology, a section of the journal Frontiers in Plant Science Edited by: Maren Müller, University of Barcelona, Spain Reviewed by: Alex Levine, Hebrew University of Jerusalem, Israel; Xuelu Wang, Huazhong Agricultural University, China; Zhong-Guang Li, Yunnan Normal University, China |
| ISSN: | 1664-462X 1664-462X |
| DOI: | 10.3389/fpls.2017.01153 |