cspB encodes a major cold shock protein in Clostridium botulinum ATCC 3502
The relative expression of three cold shock protein coding genes ( cspA, cspB and cspC) of Clostridium botulinum ATCC 3502 was studied with quantitative RT-PCR analysis following a cold shock shift from 37 °C to 15 °C. A significant increase in the relative expression of all three genes was observed...
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Published in | International journal of food microbiology Vol. 146; no. 1; pp. 23 - 30 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Netherlands
Elsevier B.V
15.03.2011
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Subjects | |
Online Access | Get full text |
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Summary: | The relative expression of three cold shock protein coding genes (
cspA,
cspB and
cspC) of
Clostridium botulinum ATCC 3502 was studied with quantitative RT-PCR analysis following a cold shock shift from 37
°C to 15
°C. A significant increase in the relative expression of all three genes was observed upon the temperature downshift. To validate these findings, single-gene insertional inactivation of
cspA,
cspB and
cspC was undertaken with the ClosTron gene knock-out system. In growth experiments, mutations in
cspB or
cspC, but not
cspA, resulted in a cold-sensitive phenotype. No growth of the
cspB mutant was observed at 15
°C over a ten day period, whereas at 20
°C the growth rate was 70% lower than that of wild type strain. The growth rate of
cspC mutant was 70% and 80% lower than the growth rate of the wild type strain at 15
°C and 20
°C, respectively. At 37
°C the growth of
cspB mutant did not differ from, but the growth rate of
cspC mutant was 30% lower than, that of the wild type strain. The
cspA mutant grew somewhat faster than the wild type strain at all studied temperatures. Since the inactivation of
cspB resulted in the most prominent defect in growth at low temperatures, we suggest that
cspB encodes the major cold shock protein of
C. botulinum ATCC 3502. Understanding the mechanisms behind cold tolerance of
C. botulinum helps to evaluate the safety risks this foodborne pathogen poses in the modern food industry.
► Expression of
csp genes increased after cold shock in
C. botulinum ATCC 3502. ► Inactivation of
cspB caused the most prominent growth defects at low temperature. ► The growth rate of
cspC mutant was decreased also at optimum temperature. ►
cspA mutant grew equally well at all temperatures. ► CspB is the major cold shock protein in
C. botulinum ATCC 3502. |
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Bibliography: | http://dx.doi.org/10.1016/j.ijfoodmicro.2011.01.033 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0168-1605 1879-3460 |
DOI: | 10.1016/j.ijfoodmicro.2011.01.033 |