Composition and antioxidant and antimicrobial activity of the essential oil and extracts of Stachys inflata Benth from Iran

• Published in: Food chemistry Vol. 119; no. 2; pp. 452 - 458
• Main Authors: Ebrahimabadi, Abdolrasoul H., Ebrahimabadi, Ebrahim H., Djafari-Bidgoli, Zahra, Kashi, Fereshteh Jookar, Mazoochi, Asma, Batooli, Hossein
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.03.2010; [Amsterdam]: Elsevier Science; Elsevier
• Subjects: Antimicrobial activity; antimicrobial properties; Antioxidant activity; Aroma and flavouring agent industries; Biological and medical sciences; chemical constituents of plants; Essential oil; essential oils; Extract; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; hexenal; Linalool; phenolic compounds; plant extracts; polar compounds; spathulenol; Stachys; Stachys inflata; terpineol; Total phenolic; α-Terpineol; Iran; Antimicrobial activity; Stachys inflata; Antioxidant activity; Linalool; Total phenolic; Essential oil; Extract; α-Terpineol; Phenols; Chemical composition; Antioxidant; Antimicrobial agent
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2009.06.037

Composition and antioxidant and antimicrobial activities of essential oil and methanol extract polar and nonpolar subfractions of Stachys inflata were determined. GC and GC/MS analyse of the essential oil showed 45 constituents representing 95.46% of the oil, the major components linalool (28.55%), α-terpineol (9.45%), spathulenol (8.37%) and (2 E)-hexenal (4.62%) constituted 50.99% of it. Essential oil and extracts were also tested for their antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene/linoleic acid assays. In the DPPH test, IC 50 value for the polar subfraction was 89.50 μg/ml, indicating an antioxidant potency of about 22% of that of butylated hydroxytoluene (IC 50 = 19.72 μg/ml) for this extract. In β-carotene/linoleic acid assay, the best inhibition belonged to the nonpolar subfraction (77.08%). Total phenolic content of the polar and nonpolar extract subfractions was 5.4 and 2.8% (w/w), respectively. The plant also showed a week antimicrobial activity against three strains of tested microorganisms. Linalool and α-terpineol were also tested as major components of the oil and showed no antioxidant but considerable antimicrobial activities.