Production of polyhydroxybutyrate by the marine photosynthetic bacterium Rhodovulum sulfidophilum P5

• Published in: Chinese journal of oceanology and limnology Vol. 30; no. 4; pp. 620 - 626
• Main Author: 才金玲 魏莹 赵宇鹏 潘光华 王广策
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.07.2012; SP Science Press; Springer Nature B.V
• Subjects: Accumulation; Ammonium; Ammonium chloride; Ammonium compounds; Bacteria; Biology; Biomass; biomass production; Carbon; Carbon sources; cell growth; Chlorides; Dry weight; Earth and Environmental Science; Earth Sciences; Growth; Limnology; Malic acid; Marine; Nitrogen; Nitrogen sources; Oceanography; Peptones; PHB; Photosynthesis; Polyesters; Polyhydroxybutyrate; Polyhydroxybutyric acid; Pyruvic acid; Rhodovulum sulfidophilum; salinity; Seawater; Sodium; Sodium chloride; Sodium pyruvate; Strain; Weight; 丙酮酸钠; 海洋光合细菌; 生产; 生物量浓度; 细胞生长; 细菌培养; 聚羟基丁酸酯; polyhydroxybutyrate; nitrogen source; carbon source; carbon to nitrogen molar ratio
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-012-1132-9

The effects of different NaCl concentrations, nitrogen sources, carbon sources, and carbon to nitrogen molar ratios on biomass accumulation and polyhydroxybutyrate （PHB） production were studied in batch cultures of the marine photosynthetic bacterium Rhodovulum sulfidophilum P5 under aerobic-dark conditions. The results show that the accumulation of PHB in strain P5 is a growth-associated process. Strain P5 had maximum biomass and PHB accumulation at 2%-3% NaCl, suggesting that the bacterium can maintain growth and potentially produce PHB at natural seawater salinity. In the nitrogen source test, the maximum biomass accumulation （8.10±0.09 g/L） and PHB production （1.11±0.13 g/L and 14.62%±2.25% of the cell dry weight） were observed when peptone and ammonium chloride were used as the sole nitrogen source. NH~-N was better for PHB production than other nitrogen sources. In the carbon source test, the maximum biomass concentration （7.65±0.05 g/L） was obtained with malic acid as the sole carbon source, whereas the maximum yield of PHB （5.03±0.18 g/L and 66.93%± 1.69% of the cell dry weight） was obtained with sodium pyruvate as the sole carbon source. In the carbon to nitrogen ratios test, sodium pyruvate and ammonium chloride were selected as the carbon and nitrogen sources, respectively. The best carbon to nitrogen molar ratio for biomass accumulation （8.77±0.58 g/L） and PHB production （6.07±0.25 g/L and 69.25%±2.05% of the cell dry weight） was 25. The results provide valuable data on the production of PHB by R. sulfidophilum P5 and further studies are on-going for best cell growth and PHB yield.