Distinct effects of methamphetamine on autophagy–lysosome and ubiquitin–proteasome systems in HL-1 cultured mouse atrial cardiomyocytes

• Published in: Toxicology (Amsterdam) Vol. 312; pp. 74 - 82
• Main Authors: Funakoshi-Hirose, Izumi, Aki, Toshihiko, Unuma, Kana, Funakoshi, Takeshi, Noritake, Kanako, Uemura, Koichi
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 04.10.2013
• Subjects: Animals; Autophagy; Autophagy - drug effects; cardiomyocytes; Cells, Cultured; Central Nervous System Stimulants - toxicity; drugs; Emergency; genes; granules; Heme Oxygenase-1 - physiology; HL-1; ligases; light microscopy; Lysosome; lysosomes; Lysosomes - drug effects; Methamphetamine; Methamphetamine - toxicity; Mice; muscles; Myocytes, Cardiac - drug effects; Myocytes, Cardiac - metabolism; myosin heavy chains; NF-E2-Related Factor 2 - physiology; pathophysiology; phenotype; precipitin tests; Proteasome; Proteasome Endopeptidase Complex - physiology; protein degradation; proteomics; quantitative polymerase chain reaction; sarcomeres; toxicology; transcription factors; Ubiquitin; Ubiquitin - metabolism; ubiquitination; vacuoles; Western blotting; Ubiquitin; MuRF1; Lysosome; ALS; MHC; HL-1; HO-1; Autophagy; lysosome-associated membrane protein1; GFP; microtubules-associated protein light chain3; LAMP1; methamphetamine; Nrf2; UPS; autophagy–lysosome system; SnPP; LC3; Proteasome; nuclear factor erythroid-2 related factor; myosin heavy chain; tin protoporphyrin; muscle ring finger1; ubiquitin–proteasome system; heme oxygenase-1; green fluorescent protein; MAP; Methamphetamine
• ISSN: 0300-483X; 1879-3185
• DOI: 10.1016/j.tox.2013.07.016

Highlights • The psychostimulant drug methamphetamine is also known to cause cardiovascular injuries. • Methamphetamine cardiotoxicity was examined using HL-1 mouse atrial cardiomyocytes. • Methamphetamine impairs the autophagy–lysosome protein degradation system. • Methamphetamine causes myosin heavy chain degradation by the ubiquitin–proteasome system.