Neuromedin U receptor 1 expression in the rat endocrine pancreas and evidence suggesting neuromedin U suppressive effect on insulin secretion from isolated rat pancreatic islets
Neuromedin U (NmU) is a regulatory peptide found in significant concentrations in both the brain and gut of the rat and is named according to its ability to powerfully contract the uterus. Two types of NmU receptors were recently identified and subsequent studies evidenced NmU involvement in the reg...
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Published in | International journal of molecular medicine Vol. 18; no. 5; pp. 951 - 955 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Greece
D.A. Spandidos
01.11.2006
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Subjects | |
Online Access | Get full text |
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Summary: | Neuromedin U (NmU) is a regulatory peptide found in significant concentrations
in both the brain and gut of the rat and is named according to its ability to
powerfully contract the uterus. Two types of NmU receptors were recently identified
and subsequent studies evidenced NmU involvement in the regulation of energy homeostasis.
Such a role of neuromedin U suggests that a polypeptide may also be involved in
the regulation of adipoinsular axis function. Therefore in the present study we
examined the expression of NmU receptors in pancreatic islets using RT-PCR and
Western blotting analysis. We also investigated the role of NmU in regulation
of insulin secretion in vitro using isolated pancreatic islets. We have confirmed
that NmUR1 but not NmUR2 is specifically expressed in isolated rat pancreatic
islets. In all tested doses (1, 10, 100 nmol/l) NmU dose- dependently decreased
insulin output by isolated pancreatic islets. These inhibitory effects of NmU
on insulin secretion may suggest the involvement of NmU in regulating the pancreatic
branch of adipoinsular axis function. Thus, NmU can be included in that group
of anorectic peptides, which are also involved in the regulation of insulin secretion. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1107-3756 1791-244X |
DOI: | 10.3892/ijmm.18.5.951 |