Gastroprotective potential of frutalin, a d-galactose binding lectin, against ethanol-induced gastric lesions

• Published in: Fitoterapia Vol. 83; no. 3; pp. 604 - 608
• Main Authors: de Vasconcellos Abdon, Ana Paula, Coelho de Souza, Greicy, Noronha Coelho de Souza, Lílian, Prado Vasconcelos, Renata, Araújo Castro, Carolina, Moreira Guedes, Marjorie, Pereira Lima Júnior, Roberto César, de Azevedo Moreira, Renato, de Oliveira Monteiro-Moreira, Ana Cristina, Rolim Campos, Adriana
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2012
• Subjects: Absolute ethanol; adrenergic receptors; Animals; Anti-Ulcer Agents - pharmacology; Anti-Ulcer Agents - therapeutic use; Antioxidants - metabolism; Antioxidants - pharmacology; Antioxidants - therapeutic use; Artocarpus incisa; Biomarkers - metabolism; calcium channels; Capsaicin - analogs & derivatives; Capsaicin - pharmacology; cimetidine; Cimetidine - therapeutic use; Ethanol; Frutalin; Galactose - metabolism; Galectins - pharmacology; Galectins - therapeutic use; Gastric Mucosa - drug effects; Gastric Mucosa - pathology; Gastroprotection; Glutathione - metabolism; Glyburide - pharmacology; indomethacin; Indomethacin - pharmacology; intragastric administration; lectins; Male; Malondialdehyde - metabolism; medicine; Mice; Mice, Inbred Strains; NG-Nitroarginine Methyl Ester - pharmacology; nitric oxide; oxidative stress; Oxidative Stress - drug effects; Phytotherapy; Plant Extracts - pharmacology; Plant Extracts - therapeutic use; potassium channels; prostaglandins; sodium chloride; Stomach Ulcer - chemically induced; Stomach Ulcer - drug therapy; Stomach Ulcer - metabolism; stomach ulcers; Gastroprotection; Frutalin; Artocarpus incisa; Absolute ethanol
• ISSN: 0367-326X; 1873-6971
• DOI: 10.1016/j.fitote.2012.01.005

The present study was designed to verify whether frutalin (FTL) affords gastroprotection against the ethanol-induced gastric damage and to examine the underlying mechanism(s). Gastric damage was induced by intragastric administration of 0.2ml of ethanol (96%). Mice in groups were pretreated with FTL (0.25, 0.5 and 1mg/kg; i.p.), cimetidine (100mg/kg; p.o.), or vehicle (0.9% of NaCl, 10mL/kg; p.o.), 30min before ethanol administration. They were sacrificed 30min later, the stomachs excised, and the mucosal lesion area (mm2) measured by planimetry. Gastroprotection was assessed in relation to inhibition of gastric lesion area. To study the gastroprotective mechanism(s), its relations to capsaicin-sensitive fibers, endogenous prostaglandins, nitric oxide, sulphydryls, ATP-sensitive potassium channels, adrenoceptors, opioid receptors and calcium channels were analyzed. Treatments effects on ethanol-associated oxidative stress markers GSH and MDA were measured in gastric tissue. FTL afforded a dose-unrelated gastroprotection against the ethanol damage. However, it failed to prevent the ethanol-induced changes in the levels of GSH and MDA. It was observed that the gastroprotection by FTL was greatly reduced in animals pretreated with capsazepine, indomethacin, L-NAME or glibenclamide. Considering the results, it is suggested that the FTL could probably be a good therapeutic agent for the development of new medicine for the treatment of gastric ulcer. [Display omitted]