Citrus peel influences the production of an extracellular naringinase by Staphylococcus xylosus MAK2 in a stirred tank reactor

• Published in: Applied microbiology and biotechnology Vol. 89; no. 3; pp. 715 - 722
• Main Authors: Puri, Munish, Kaur, Aneet, Barrow, Colin J., Singh, Ram Sarup
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.02.2011; Springer; Springer Nature B.V
• Subjects: beta-Glucosidase - metabolism; Biological and medical sciences; Biomedical and Life Sciences; Bioreactors; Biotechnologically Relevant Enzymes and Proteins; Biotechnology; Biotechnology - methods; Calcium - metabolism; Cations, Divalent - metabolism; Citrus; Citrus - metabolism; Citrus fruits; Culture Media - chemistry; Enzymatic activity; Enzyme Activators - metabolism; Enzymes; Fermentation; Flavonoids; Food processing industry; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Hydrogen-Ion Concentration; Laboratories; Life Sciences; Membrane reactors; Methods. Procedures. Technologies; Microbial Genetics and Genomics; Microbiology; Multienzyme Complexes - metabolism; Optimization; Reactors; Soil Microbiology; Staphylococcus - enzymology; Staphylococcus - isolation & purification; Studies; Temperature; Various methods and equipments; Bioreactor; Citrus peel; Naringin; Flavonoid; Rhamnosidase; Staphylococcus xylosus; Rutaceae; Extracellular; Stirred tank reactor; Citrus; Dicotyledones; Angiospermae; Production; Bacteria; Micrococcales; Micrococcaceae; Spermatophyta
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-010-2897-4

Staphylococcus xylosus MAK2, Gram-positive coccus, a nonpathogenic member of the coagulase-negative Staphylococcus family was isolated from soil and used to produce naringinase in a stirred tank reactor. An initial medium at pH 5.5 and a cultivation temperature of 30°C was found to be optimal for enzyme production. The addition of Ca +2 caused stimulation of enzyme activity. The effect of various physico-chemical parameters, such as pH, temperature, agitation, and inducer concentration was studied. The enzyme production was enhanced by the addition of citrus peel powder (CPP) in the optimized medium. A twofold increase in naringinase production was achieved using different technological combinations. The process optimization using technological combinations allowed rapid optimization of large number of variables, which significantly improved enzyme production in a 5-l reactor in 34 h. An increase in sugar concentration (15 g l −1 ) in the fermentation medium further increased naringinase production (8.9 IU ml −1 ) in the bioreactor. Thus, availability of naringinase renders it attractive for potential biotechnological applications in citrus processing industry.