Development and characterisation of a Brassica carinata inbred line incorporating genes for low glucosinolate content from B. juncea

• Published in: Euphytica Vol. 164; no. 2; pp. 365 - 375
• Main Authors: Márquez-Lema, Angustias, Fernández-Martínez, José M, Pérez-Vich, Begoña, Velasco, Leonardo
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.11.2008; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Brassica carinata; Brassica juncea; chemical constituents of plants; chromosome mapping; Chromosomes; Classical and quantitative genetics. Population genetics. Molecular genetics; Fundamental and applied biological sciences. Psychology; Generalities. Genetics. Plant material; genes; genetic markers; Genetics and breeding of economic plants; Genotype & phenotype; glucosinolates; inbred lines; Inbreeding; Interspecific and intergeneric hybridization, introgressions; interspecific hybridization; introgression; Life Sciences; microsatellite repeats; Molecular genetics; plant breeding; Plant breeding: fundamental aspects and methodology; plant development; Plant Genetics and Genomics; plant growth; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; seeds; sinigrin; Interspecific crosses; Sinigrin; Phenotypic traits; Simple sequence repeats (SSRs); Sinigrin, Interspecific crosses, Phenotypic traits; Glucosinolate; Low content; Phenotype; Vegetable crop; Gene; Cruciferae; Dicotyledones; Microsatellite DNA; Angiospermae; Genetic improvement; Interspecific hybridization; Spermatophyta; Brassica carinata; Pure line; Brassica carinata, Brassica juncea
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-008-9678-5

The presence of high levels of sinigrin in the seeds represents a serious constraint for the commercial utilisation of Ethiopian mustard (Brassica carinata A. Braun) meal. The objective of this research was the introgression of genes for low glucosinolate content from B. juncea into B. carinata. BC₁F₁ seed from crosses between double zero B. juncea line Heera and B. carinata line N2-142 was produced. Simultaneous selection for B. carinata phenotype and low glucosinolate content was conducted from BC₁F₂ to BC₁F₄ plant generations. Forty-three BC₁F₄ derived lines were selected and subject to a detailed phenotypic and molecular evaluation to identify lines with low glucosinolate content and genetic proximity to B. carinata. Sixteen phenotypic traits and 80 SSR markers were used. Eight BC₁F₄ derived lines were very close to N2-142 both at the phenotypic and molecular level. Three of them, with average glucosinolate contents from 52 to 61 micromoles g⁻¹, compared to 35 micromoles g⁻¹ for Heera and 86 micromoles g⁻¹ for N2-142, were selected and evaluated in two additional environments, resulting in average glucosinolate contents from 43 to 56 micromoles g⁻¹, compared to 29 micromoles g⁻¹ for Heera and 84 micromoles g⁻¹ for N2-142. The best line (BCH-1773), with a glucosinolate profile made up of sinigrin (>95%) and a chromosome number of 2n = 34, was further evaluated in two environments (field and pots in open-air conditions). Average glucosinolate contents over the four environments included in this research were 42, 31 and 74 micromoles g⁻¹ for BCH-1773, Heera and N2-142, respectively. These are the lowest stable levels of glucosinolates reported so far in B. carinata.