Higher frequencies of circulating ICOS+, IL-21+ T follicular helper cells and plasma cells in patients with new-onset membranous nephropathy
Background: T follicular helper (TFH) cells and B cells are known to regulate humoral immune responses. This study is aimed at examining the putative contribution of different subsets of circulating of TFH cells and B cells to membranous nephropathy (MN). Methods: A total of 45 MN patients and 19 he...
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Published in | Autoimmunity (Chur, Switzerland) Vol. 50; no. 8; pp. 458 - 467 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Taylor & Francis
17.11.2017
Taylor & Francis Group |
Subjects | |
Online Access | Get full text |
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Summary: | Background: T follicular helper (TFH) cells and B cells are known to regulate humoral immune responses. This study is aimed at examining the putative contribution of different subsets of circulating of TFH cells and B cells to membranous nephropathy (MN).
Methods: A total of 45 MN patients and 19 healthy controls (HCs) were examined for the number of TFH cells and B cells by flow cytometry. The level of 24-h urinary protein and eGFR were calculated, and the level of serum cytokines was examined. The potential association among these measures was analyzed.
Results: Compared to the HCs, MN patients had significantly higher numbers of circulating CD4
+
CXCR5
+
, CD4
+
CXCR5
+
ICOS
+
, CD4
+
CXCR5
+
CD154
+
, CD4
+
CXCR5
+
IL-21
+
, and CD4
+
CXCR5
+
CD28
+
TFH cells, as well as IgD
+
CD27
−
CD19
+
and CD138
+
CD19
+
B cells. However, the number of IgD
+
CD27
+
CD19
+
B cells was significantly lower in MN patients than in the HC. The levels of serum IL-21, IL-2, IL-4, IL-10, IL-17A, and IFN-γ were significantly higher in MN patients than in the HC. Furthermore, the numbers of CD4
+
CXCR5
+
, CD4
+
CXCR5
+
ICOS
+
, CD4
+
CXCR5
+
CD154
+
, CD4
+
CXCR5
+
IL-21
+
, CD4
+
CXCR5
+
CD28
+
TFH cells, CD138
+
CD19
+
B cells, and the level of sera IL-21 were negatively correlated with the values of eGFR, but positively correlated with the levels of 24-h urinary proteins. Following treatment, the numbers of CD4
+
CXCR5
+
, CD4
+
CXCR5
+
ICOS
+
, CD4
+
CXCR5
+
CD154
+
, CD4
+
CXCR5
+
IL-21
+
, CD4
+
CXCR5
+
CD28
+
TFH cells, CD138
+
CD19
+
B cells, and the levels of IL-21 were significantly reduced. In contrast, IL-4 and IL-10 levels were noticeably increased after treatment.
Conclusions: Data suggest that activated TFH and plasma cells may contribute to the pathogenesis of MN. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0891-6934 1607-842X |
DOI: | 10.1080/08916934.2017.1385775 |