Occurrence, Genotyping, and Antibiotic Susceptibility of Cronobacter spp. in Drinking Water and Food Samples from Northeast China

• Published in: Journal of food protection Vol. 81; no. 3; pp. 456 - 460
• Main Authors: Fei, Peng, Jiang, Yichao, Gong, Shaoying, Li, Ran, Jiang, Yan, Yuan, Xiujuan, Wang, Ziyuan, Kang, Huaibin, Ali, Md Aslam
• Format: Journal Article
• Language: English
• Published: United States Elsevier Limited 01.03.2018
• Subjects: Adults; Ampicillin; Antibiotic resistance; Antibiotics; Baby foods; Bacteria; Cefotaxime; Cephalothin; Chloramphenicol; Ciprofloxacin; Cookies; Cronobacter; Cronobacter sakazakii; Deoxyribonucleic acid; DNA; Drinking water; Drug resistance; Enterobacter; Food; Food production; Food safety; Genes; Genomics; Genotyping; Gentamicin; Hazards; Infants; Infections; Laboratories; Meningitis; Meropenem; Multilocus sequence typing; Noodles; Opportunist infection; Pathogens; Phylogenetics; Piperacillin; Piperacillin-tazobactam; Pork; Rice; Species; Strains (organisms); Sulbactam; Sulfamethoxazole; Tazobactam; Trimethoprim; Trimethoprim-sulfamethoxazole; Vegetables; Beijing China; China; Cronobacter spp; Genotyping; Occurrence; Antibiotic susceptibility
• ISSN: 0362-028X; 1944-9097
• DOI: 10.4315/0362-028X.JFP-17-326

Cronobacter species (formerly Enterobacter sakazakii) are emerging opportunistic bacterial pathogens that can infect both infants and adults. This study was conducted to isolate and genotype diverse Cronobacter species from drinking water, chilled fresh pork, powdered infant formula, instant noodles, cookies, fruits, vegetables, and dishes in Northeast China and to evaluate the antibiotic resistance and susceptibility of the isolates. Thirty-four Cronobacter strains were isolated and identified: 21 C. sakazakii isolates (61.8%), 10 C. malonaticus isolates (29.4%), 2 C. dublinensis isolates (5.9%), and 1 C. turicensis isolate (2.9%). These isolates were further divided into 15 sequence types (STs) by multilocus sequence typing. C. sakazakii ST4 (10 isolates, 29.4%), ST1 (3 isolates, 8.8%), and ST8 (3 isolates, 8.8%) and C. malonaticus ST7 (four isolates, 11.8%) were dominant. Antibiotic susceptibility testing indicated that all 34 Cronobacter isolates were susceptible to ampicillin-sulbactam, cefotaxime, ciprofloxacin, gentamicin, meropenem, tetracycline, piperacillin-tazobactam, and trimethoprim-sulfamethoxazole, 88.2% were susceptible to chloramphenicol, and 67.6% were resistant to cephalothin. The results of this study enhance knowledge about genotyping and antibiotic resistance of these Cronobacter species and could be used to prevent potential hazards caused by these strains in drinking water and various food products.