LATERAL ORGAN BOUNDARIES DOMAIN transcription factors direct callus formation in Arabidopsis regeneration

The remarkable regeneration capability of plant tissues or organs under culture conditions has underlain an extensive practice for decades. The initial step in plant in vitro regeneration often involves the induction of a pluripotent cell mass termed callus, which is driven by the phytohormone auxin...

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Bibliographic Details
Published inCell research Vol. 22; no. 7; pp. 1169 - 1180
Main Authors Fan, Mingzhu, Xu, Chongyi, Xu, Ke, Hu, Yuxin
Format Journal Article
LanguageEnglish
Published England Nature Publishing Group 01.07.2012
Subjects
Arabidopsis - genetics
Arabidopsis - metabolism
Arabidopsis - physiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Gene Expression Regulation, Plant - genetics
Gene Expression Regulation, Plant - physiology
Indoleacetic Acids - metabolism
Original
Reproduction - genetics
Reproduction - physiology
Transcription Factors - genetics
Transcription Factors - metabolism
再生能力
发光二极管
器官
愈伤组织形成
拟南芥
诱导培养基
转录因子
边界域
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Summary:The remarkable regeneration capability of plant tissues or organs under culture conditions has underlain an extensive practice for decades. The initial step in plant in vitro regeneration often involves the induction of a pluripotent cell mass termed callus, which is driven by the phytohormone auxin and occurs via a root development pathway. However, the key molecules governing callus formation remain unknown. Here we demonstrate that Arabidopsis LATERAL ORGAN BOUNDARIES DOMAIN （LBD）/ASYMMETRIC LEAVES2-LIKE （ASL） transcription factors are involved in the control of callus formation program. The four LBD genes downstream of AUXIN RESPONSE FACTORs （ARFs）, LBD16, LBD17, LBD18 and LBD29, are rapidly and dramatically induced by callus-inducing medium （CIM） in multiple organs. Ectopic expression of each of the four LBD genes in Arabidopsis is sufficient to trigger spontaneous callus formation without exogenous phytohormones, whereas suppression of LBD function inhibits the callus formation induced by CIM. Moreover, the callus triggered by LBD resembles that induced by CIM by characteristics of ectopically activated root meristem genes and efficient regeneration capacity. These findings define LBD transcription factors as key regulators in the callus induction process, thereby establishing a molecular link between auxin signaling and the plant regeneration program.
Bibliography:LBD; callus formation; auxin; regeneration; Arabidopsis
The remarkable regeneration capability of plant tissues or organs under culture conditions has underlain an extensive practice for decades. The initial step in plant in vitro regeneration often involves the induction of a pluripotent cell mass termed callus, which is driven by the phytohormone auxin and occurs via a root development pathway. However, the key molecules governing callus formation remain unknown. Here we demonstrate that Arabidopsis LATERAL ORGAN BOUNDARIES DOMAIN （LBD）/ASYMMETRIC LEAVES2-LIKE （ASL） transcription factors are involved in the control of callus formation program. The four LBD genes downstream of AUXIN RESPONSE FACTORs （ARFs）, LBD16, LBD17, LBD18 and LBD29, are rapidly and dramatically induced by callus-inducing medium （CIM） in multiple organs. Ectopic expression of each of the four LBD genes in Arabidopsis is sufficient to trigger spontaneous callus formation without exogenous phytohormones, whereas suppression of LBD function inhibits the callus formation induced by CIM. Moreover, the callus triggered by LBD resembles that induced by CIM by characteristics of ectopically activated root meristem genes and efficient regeneration capacity. These findings define LBD transcription factors as key regulators in the callus induction process, thereby establishing a molecular link between auxin signaling and the plant regeneration program.
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These two authors contributed equally to this work.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2012.63