Development and validation of an inexpensive and efficient method for the extraction of Theileria orientalis DNA from blood

Theileria orientalis is an emerging bovine pathogen in Australasia. PCR-based detection methods for this parasite are sensitive but relatively expensive, partly due to costs associated with DNA extraction. An inexpensive and efficient technique was developed for the extraction of T. orientalis DNA f...

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Bibliographic Details
Published inVeterinary parasitology Vol. 212; no. 3-4; pp. 379 - 381
Main Authors Bogema, D.R., Fell, S.A., O'Rourke, B.A., Collins, D., Eamens, G.J., Jenkins, C.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.09.2015
Subjects
Animals
Cattle
Detergents
DNA extraction
DNA, Protozoan - blood
DNA, Protozoan - genetics
DPK method
Endopeptidase K
Erythrocytes
Genotypes
qPCR
Reproducibility of Results
Theileria - genetics
Theileria orientalis
Theileriasis - blood
Theileriasis - parasitology
DPK method
DNA extraction
qPCR
Genotypes
Theileria orientalis
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Summary:Theileria orientalis is an emerging bovine pathogen in Australasia. PCR-based detection methods for this parasite are sensitive but relatively expensive, partly due to costs associated with DNA extraction. An inexpensive and efficient technique was developed for the extraction of T. orientalis DNA from blood based on hypotonic erythrocyte lysis and detergent-proteinase K treatment (DPK method). The DPK method compares favourably to a commercial extraction kit when paired with a T. orientalis multiplex qPCR.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2015.08.014