Altered invertase activities of symptomatic tissues on Beet severe curly top virus (BSCTV) infected Arabidopsis thaliana

• Published in: Journal of plant research Vol. 126; no. 5; pp. 743 - 752
• Main Authors: Park, Jungan, Kim, Soyeon, Choi, Eunseok, Auh, Chung-Kyun, Park, Jong-Bum, Kim, Dong-Giun, Chung, Young-Jae, Lee, Taek-Kyun, Lee, Sukchan
• Format: Journal Article
• Language: English
• Published: Tokyo Springer Japan 01.09.2013; Springer Nature B.V
• Subjects: Arabidopsis - enzymology; Arabidopsis - genetics; Arabidopsis - virology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; Base Sequence; Beta vulgaris - virology; beta-Fructofuranosidase - genetics; beta-Fructofuranosidase - metabolism; Biomedical and Life Sciences; Carotenoids - metabolism; Cell Cycle Proteins - genetics; Chlorophyll - metabolism; Cyclins - genetics; E2F Transcription Factors - genetics; Geminiviridae - physiology; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Plant; Glucosyltransferases - metabolism; Inflorescence - enzymology; Inflorescence - genetics; Inflorescence - virology; Life Sciences; Molecular Sequence Data; Plant Biochemistry; Plant diseases; Plant Diseases - virology; Plant Ecology; Plant growth; Plant Leaves - enzymology; Plant Leaves - genetics; Plant Leaves - virology; Plant Physiology; Plant Sciences; Plant Stems - enzymology; Plant Stems - genetics; Plant Stems - virology; Plants, Genetically Modified; Regular Paper; Ribulose-Bisphosphate Carboxylase - metabolism; Sucrose - metabolism; Trees; Viruses; Beet severe curly top virus; E2F; D-type cyclin; Invertase
• ISSN: 0918-9440; 1618-0860
• DOI: 10.1007/s10265-013-0562-6

Arabidopsis thaliana infected with Beet severe curly top virus (BSCTV) exhibits systemic symptoms such as stunting of plant growth, callus induction on shoot tips, and curling of leaves and shoot tips. The regulation of sucrose metabolism is essential for obtaining the energy required for viral replication and the development of symptoms in BSCTV-infected A. thaliana . We evaluated the changed transcript level and enzyme activity of invertases in the inflorescence stems of BSCTV-infected A. thaliana . These results were consistent with the increased pattern of ribulose-1,5-bisphosphate carboxylase/oxygenase activity and photosynthetic pigment concentration in virus-infected plants to supply more energy for BSCTV multiplication. The altered gene expression of invertases during symptom development was functionally correlated with the differential expression patterns of D-type cyclins, E2F isoforms, and invertase-related genes. Taken together, our results indicate that sucrose sensing by BSCTV infection may regulate the expression of sucrose metabolism and result in the subsequent development of viral symptoms in relation with activation of cell cycle regulation.