Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera

The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, Nelumbo nucifera. All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification wa...

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Published inSaudi journal of biological sciences Vol. 27; no. 11; pp. 2883 - 2889
Main Authors Techaoei, Surachai, Jirayuthcharoenkul, Chariya, Jarmkom, Khemjira, Dumrongphuttidecha, Thisakorn, Khobjai, Warachate
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.11.2020
Elsevier
Subjects
Antimicrobial activity
Endophytic fungi
Methicillin-resistant S. aureus
N. nucifera
Original
Methicillin-resistant S. aureus
Antimicrobial activity
Endophytic fungi
N. nucifera
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Summary:The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, Nelumbo nucifera. All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification was characterized by morphological and molecular technique. Fungal isolates were screened to discover antimicrobial activities by disc diffusion method against Methicillin-resistant Staphylococcus aureus DMST20651 (MRSA). MIC and MBC for those crude fungal extracts were determined. Finally, the chemical profile of crude extract was determined by gas chromatography and mass spectrometry. Six endophytic fungi were isolated from the surface-satirized parts of N. nucifera. Based on disc diffusion assay, the highest antibacterial activity against MRSA was isolate ST9.1 identified as Aspergillus cejpii. Results demonstrated that the ethyl acetate extraction had more active fractions with MIC of 2.5 mg/ml and MBC concentration of 50.0 mg/ml. The crude extracts were developed to identify the chemical constituents by gas chromatography and mass spectrometry. The major component of crude extract of endophytic fungi was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (C11H11N5). Thus, the plant could be used in the treatment of infectious diseases caused by bacterial pathogen.
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ISSN:1319-562X
2213-7106
DOI:10.1016/j.sjbs.2020.08.037