Glycoconjugate mediated endothelial cell adhesion to Dacron polyester film

• Published in: Journal of vascular surgery Vol. 18; no. 3; pp. 486 - 494
• Main Authors: Ozaki, C.Keith, Phaneuf, Matthew D., Hong, Suchen L., Quist, William C., LoGerfo, Frank W.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: New York, NY Mosby, Inc 01.09.1993; Elsevier
• Subjects: Biological and medical sciences; Cell Adhesion - drug effects; Cells, Cultured; Endothelium, Vascular - cytology; Endothelium, Vascular - drug effects; Endothelium, Vascular - physiology; Glycoconjugates - physiology; Humans; Lectins - pharmacology; Medical sciences; Plant Lectins; Polyethylene Terephthalates; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Vascular surgery: aorta, extremities, vena cava. Surgery of the lymphatic vessels; Ethylene terephthalate polymer; Human; Lectin; Polyethylene; Endothelial cell; Prosthesis; Oligosaccharide; Cardiovascular disease; Experimental study; Adhesion; In vitro; Cell surface; Vascular disease; Leguminosae; Surgery; Dicotyledones; Blood vessel; Angiospermae; Plant origin; Biomaterial; Spermatophyta; Ulex europaeus; Biomedical engineering
• ISSN: 0741-5214; 1097-6809
• DOI: 10.1016/0741-5214(93)90267-P

Purpose: The purpose of this study was to explore new strategies for enhancing specific cell type attachment to biomaterials using immobilized lectins for cell surface glycoconjugates. The lectin Ulex europaeus I (UEA I) has a high affinity for human vascular endothelial cell surface glycoconjugates. Methods: UEA I was covalently bound to polyethylene terephthalate (Dacron) with the cross-linking agent 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride to achieve oligosaccharide-mediated endothelial cell attachment to this otherwise nonadherent surface. Results: Experiments with radiolabeled UEA I demonstrated covalent linkage of as much as 1.35 μg/cm2. The lectin binding site is available after the reaction, as demonstrated in experiments a neoglycoprotein. Adhesion studies reveal a 100-fold increase in endothelial cell attachment for the UEA I/polyethylene terephthalate surface (99.7 ± 29.6 cells/high-power field) when compared with untreated (0.7 ± 0.5), crosslinking agent (0.4 ± 0.3), and denatured UEA I (1.2 ± 1.1) control groups. Five vascular endothelial cell lines adhered to the UEA I/polyethylene terephthalate surface, whereas monocytes, smooth muscle cells, and fibroblasts did not. Conclusion: These results imply new strategies for endothelialization of prosthetic grafts and promotion of selective cell adherence to biomaterials, with emphasis on carbohydrate interactions. Moreover, this experimental system offers a model for exploring the biologic significance of the endothelial cell-UEA I ligand.