The effects of urease immobilization on the transport characteristics and protein adsorption capacity of cellulose acetate based hemodialysis membranes

• Published in: Journal of materials science. Materials in medicine Vol. 20; no. 10; pp. 2167 - 2179
• Main Authors: Mahlicli, Filiz Yasar, Altinkaya, Sacide Alsoy
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.10.2009; Springer Nature B.V
• Subjects: Adsorption - drug effects; Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Biotechnology; Cellulose - analogs & derivatives; Cellulose - chemistry; Cellulose - metabolism; Ceramics; Chemistry and Materials Science; Composites; Diffusion; Enzymes, Immobilized - chemistry; Enzymes, Immobilized - metabolism; Enzymes, Immobilized - pharmacology; Glass; Hemodialysis; Kinetics; Materials Science; Materials Testing; Membranes; Membranes, Artificial; Models, Biological; Models, Theoretical; Natural Materials; Permeability; Polymer Sciences; Protein Transport - drug effects; Proteins; Proteins - pharmacokinetics; Regenerative Medicine/Tissue Engineering; Renal Dialysis - instrumentation; Renal Dialysis - methods; Surfaces and Interfaces; Thin Films; Urease - chemistry; Urease - metabolism; Urease - pharmacology; Uric Acid; Permeation Experiment; Cellulose Acetate Membrane; Receiver Compartment; Cellulose Acetate
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-009-3776-3

In this study, cellulose acetate (CA) based hemodialysis membranes were prepared by a dry phase inversion method and the influences of urease immobilization on the clearing performance and protein adsorption capacity of the membranes were investigated. Permeation experiments have shown that modification of CA membranes with urease immobilization not only enhanced the transport rate of urea but also increased the permeation coefficients of uric acid and creatinine by changing the structure of the membrane. Furthermore, the protein adsorption capacity of the CA membranes decreased. On the other hand, the mechanical strength of the modified CA membrane did not change significantly compared with that of the unmodified one. A mathematical model was derived to determine the rate of mass transfer of urea through modified CA membranes. Model predictions along with the experimental data suggest that urease immobilization can be used as an alternative method in preparing CA based hemodialysis membranes with improved transport characteristics and biocompatibility through reduced protein adsorption capacities.