A novel qPCR protocol for the specific detection and quantification of the fuel-deteriorating fungus Hormoconis resinae

• Published in: Biofouling (Chur, Switzerland) Vol. 32; no. 6; pp. 635 - 644
• Main Authors: Martin-Sanchez, Pedro M., Gorbushina, Anna A., Kunte, Hans-Jörg, Toepel, Jörg
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.07.2016
• Subjects: Ascomycota - genetics; Ascomycota - isolation & purification; Bacteria; Bacterial corrosion; Contaminants; Corrosion; Culture; diesel biodeterioration; Diesel fuels; DNA, Fungal - genetics; Fouling; Fuel systems; Fungi; Genome, Fungal; Hormoconis resinae; Hydrocarbons - analysis; Hydrocarbons - standards; indicator; Kerosene - microbiology; microbial contamination; microbiologically influenced corrosion; Real-Time Polymerase Chain Reaction - methods; Real-time quantitative PCR; Reproducibility of Results; Risk Assessment; RNA Polymerase II - genetics; Real-time quantitative PCR; indicator; microbial contamination; fouling; microbiologically influenced corrosion; diesel biodeterioration
• ISSN: 0892-7014; 1029-2454
• DOI: 10.1080/08927014.2016.1177515

A wide variety of fungi and bacteria are known to contaminate fuels and fuel systems. These microbial contaminants have been linked to fuel system fouling and corrosion. The fungus Hormoconis resinae, a common jet fuel contaminant, is used in this study as a model for developing innovative risk assessment methods. A novel qPCR protocol to detect and quantify H. resinae in, and together with, total fungal contamination of fuel systems is reported. Two primer sets, targeting the markers RPB2 and ITS, were selected for their remarkable specificity and sensitivity. These primers were successfully applied on fungal cultures and diesel samples demonstrating the validity and reliability of the established qPCR protocol. This novel tool allows clarification of the current role of H. resinae in fuel contamination cases, as well as providing a technique to detect fungal outbreaks in fuel systems. This tool can be expanded to other well-known fuel-deteriorating microorganisms.