Metabolic adaptations to arsenic-induced oxidative stress in Pteris vittata L and Pteris ensiformis L

• Published in: Plant science (Limerick) Vol. 170; no. 2; pp. 274 - 282
• Main Authors: Singh, Nandita, Ma, Lena Q., Srivastava, Mrittunjai, Rathinasabapathi, Bala
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.02.2006; Elsevier Science
• Subjects: Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; antioxidants; Arsenic; Ascorbate; ascorbic acid; Biological and medical sciences; carotenoids; chlorophyll; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Glutathione; hydrogen peroxide; Hyperaccumulator; hyperaccumulators; oxidative stress; plant biochemistry; plant physiology; plant pigments; plant proteins; plant stress; pollutants; protein content; Pteris ensiformis; Pteris ensiformis L; Pteris vittata; Pteris vittata L; reactive oxygen species; signal transduction; soil pollution; Thiobarbituric acid-reactive substances; Varietal selection. Specialized plant breeding, plant breeding aims; Thiobarbituric acid-reactive substances; Arsenic; Hyperaccumulator; Pteris vittata L; Ascorbate; Pteris ensiformis L; Glutathione; Chlorophyll; Oxidative stress; Tolerance; Pteridophyta; Antioxidant; Hydroponic cultivation; Carotenoid; Adaptation; Filicineae
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/j.plantsci.2005.08.013

This study examined the metabolic adaptations of Pteris vittata L, an arsenic hyperaccumulator, under arsenic stress as compared to Pteris ensiformis, a non-arsenic hyperaccumulator. Both plants were grown hydroponically in 20% Hoagland medium in controlled conditions and were treated with 0, 133 or 267 μM arsenic as sodium arsenate for 1, 5 or 10 d. The fern fronds were analysed for differences in oxidative stress and antioxidant capacities after arsenic exposure. Upon exposure to 133 μM arsenic, concentrations of chlorophyll, protein and carotenoids increased in P. vittata whereas they decreased in P. ensiformis. The H 2O 2 and TBARs concentrations were greater in P. ensiformis than P. vittata in all treatments, indicating greater production of reactive oxygen species (ROS) by P. ensiformis. The levels of ascorbate and glutathione, and their reduced/oxidized ratios in the fronds of P. vittata of the control was much greater than P. ensiformis indicating that P. vittata has an inherently greater antioxidant potential than P. ensiformis. The lower levels of antioxidant compounds (ascorbate, carotenoids and glutathione) in P. ensiformis than P. vittata are consistent with its greater exposure to ROS and lower scavenging ability. The results together indicate that protection from oxidative damage by a greater level of ascorbate–glutathione pool is involved in the arsenic-tolerance in arsenic-hyperaccumulator P. vittata.