Genetic diversity and population structure of Eleutheronema rhadinum in the East and South China Seas revealed in mitochondrial COI sequences

• Published in: Chinese journal of oceanology and limnology Vol. 31; no. 6; pp. 1276 - 1283
• Main Author: 孙鑫序 徐冬冬 楼宝 张涛 辛俭 郭垚示 马世磊
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.11.2013; Springer Berlin Heidelberg; Springer Nature B.V
• Subjects: Animal populations; Biology; Branches; China; coastal water; Coastal waters; Cytochrome-c oxidase; Cytochromes; Earth and Environmental Science; Earth Sciences; Eleutheronema; Eleutheronema rhadinum; Evolution; Exploitation; Fish; Fisheries; genes; Genetic diversity; Genetic variation; Haplotypes; Marine; Mitochondrial DNA; Nucleotides; Oceanography; Population genetics; Population growth; Population structure; Population studies; Populations; Resource management; Sustainability; variance; Variance analysis; Wildlife conservation; 东海; 中国南海; 可持续开发利用; 序列; 种群结构; 线粒体; 细胞色素C氧化酶; 遗传多样性; South China Sea; East China Sea; ISEW, South China Sea; China, People's Rep., Zhejiang Prov., Zhoushan Qundao Is., Zhoushan Dao I., Zhoushan; China, People's Rep; China; population structure; cytochrome c oxidase subunit I (COI); genetic diversity
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-013-3005-2

Eleutheronema rhadinum is a potential commercial fisheries species and is subject to intense exploitation in China. Knowledge on the population structure of E. rhadinum in Chinese coastal waters, which is important for sustainable exploitation and proper resource management, is lacking. In the present study, the genetic diversity and population structure of E. rhadinum were evaluated using a 564-base pair fragment of the mitochondrial cytochrome c oxidase subunit I （COl） gene. A total of 76 specimens were collected from three localities around the East （Qidong and Zhoushan） and South China Seas （Zhuhai）. Among these individuals, nine polymorphic sites were detected and 1 l distinct haplotypes were defined. High levels ofhaplotype diversity （h=0.759i0.035） and low levels of nucleotide diversity （re=0.001 98i0.003 26） were observed in these populations, Hierarchical analysis of molecular variance （AMOVA） indicated that 96.72% of the genetic variation occurred within the populations, whereas 3.28% occurred among populations. No significant genealogical branches or clusters were recognized on the neighbor-joining tree. Intra-group variation among populations was significant （~0~t=0.032 85, P〈0.01）. These results suggest that E. rhadinum populations in the East and South China Seas have developed divergent genetic structures. Tests of neutral evolution and mismatch distribution suggest that E. rhadinum may have experienced a population expansion. The present study provides basic information for the conservation and sustainable exploitation of this species.