Growth of the Mammalian Golgi Apparatus during Interphase

During the cell cycle, genetic materials and organelles are duplicated to ensure that there is sufficient cellular content for daughter cells. While Golgi growth in interphase has been observed in lower eukaryotes, the elaborate ribbon structure of the mammalian Golgi apparatus has made it challengi...

Full description

Saved in:
Bibliographic Details
Published inMolecular and cellular biology Vol. 36; no. 18; pp. 2344 - 2359
Main Authors Sin, Alex T.-W., Harrison, Rene E.
Format Journal Article
LanguageEnglish
Published United States Taylor & Francis 01.09.2016
American Society for Microbiology
Subjects
Online AccessGet full text

Cover

Loading…
More Information
Summary:During the cell cycle, genetic materials and organelles are duplicated to ensure that there is sufficient cellular content for daughter cells. While Golgi growth in interphase has been observed in lower eukaryotes, the elaborate ribbon structure of the mammalian Golgi apparatus has made it challenging to monitor. Here we demonstrate the growth of the mammalian Golgi apparatus in its protein content and volume during interphase. Through ultrastructural analyses, physical growth of the Golgi apparatus was revealed to occur by cisternal elongation of the individual Golgi stacks. By examining the timing and regulation of Golgi growth, we established that Golgi growth starts after passage through the cell growth checkpoint at late G 1 phase and continues in a manner highly correlated with cell size growth. Finally, by identifying S6 kinase 1 as a major player in Golgi growth, we revealed the coordination between cell size and Golgi growth via activation of the protein synthesis machinery in early interphase.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Citation Sin AT-W, Harrison RE. 2016. Growth of the mammalian Golgi apparatus during interphase. Mol Cell Biol 36:2344–2359. doi:10.1128/MCB.00046-16.
ISSN:1098-5549
0270-7306
1098-5549
DOI:10.1128/MCB.00046-16