The influence of salinity on toxicological effects of arsenic in digestive gland of clam Ruditapes philippinarum using metabolomics

• Published in: Chinese journal of oceanology and limnology Vol. 31; no. 2; pp. 345 - 352
• Main Author: 吉成龙 吴惠丰 刘小莉 赵建民 于君宝 尹秀丽
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.03.2013; SP Science Press; Springer Nature B.V
• Subjects: Adenosine; Adenosine diphosphate; adenosine triphosphate; ADP; Alanine; Amino acids; Arsenic; ATP; Biomarkers; Chain branching; clams; Contaminants; Digestive glands; Dilution; Earth and Environmental Science; Earth Sciences; Exposure; Glands; glucose; glutamic acid; Glutamine; Glycogen; Glycogens; Intertidal environment; Intertidal zone; Marine; Marine molluscs; Marine pollution; Metabolic response; Metabolism; Metabolomics; Mollusks; NMR; Nuclear magnetic resonance; nuclear magnetic resonance spectroscopy; Oceanography; Phosphocholine; Proline; Ruditapes philippinarum; Salinity; Salinity effects; Seawater; succinic acid; Taurine; Threonine; Toxicology; 代谢反应; 支链氨基酸; 毒理效应; 海水盐度; 消化腺; 琥珀酸盐; 砷; 菲律宾蛤仔; salinity; toxicological effects; arsenic; metabolomics; nuclear magnetic resonance (NMR)
• ISSN: 0254-4059; 2096-5508; 1993-5005; 2523-3521
• DOI: 10.1007/s00343-013-2124-0

Ruditapes philippinarum, a clam that thrives in intertidal zones of various salinities, is a useful biomonitor to marine contaminants. We investigated the influence of dilution to 75% and 50% of normal seawater salinity （31.1） on the responses of the digestive gland of R. philippinarum to arsenic exposure （20 μg/L）, using nuclear magnetic resonance （NMR）-based metabolomics. After acute arsenic exposure for 48 h, salinity-dependent differential metabolic responses were detected. In normal seawater, arsenic exposure increased the concentrations of branched-chain amino acids, and of threonine, proline, phosphocholine and adenosine, and it decreased the levels of alanine, hypotaurine, glucose, glycogen and ATP in the digestive glands. Differential changes in metabolic biomarkers observed at lower salinity （-23.3） included elevation of succinate, taurine and ATP, and depletion of branched-chain amino acids, threonine and glutamine. Unique effects of arsenic at the lowest salinity （-15.6） included down-regulation of glutamate, succinate and ADP, and up-regulation ofphosphocholine. We conclude that salinity influences the metabolic responses of this clam to arsenic.