Development of in situ-forming hydrogels for hemorrhage control

• Published in: Journal of materials science. Materials in medicine Vol. 20; no. 8; pp. 1753 - 1762
• Main Authors: Peng, Henry T., Shek, Pang N.
• Format: Journal Article
• Language: English
• Published: Boston Springer US 01.08.2009; Springer Nature B.V
• Subjects: Biomaterials; Biomedical Engineering and Bioengineering; Biomedical materials; Blood Coagulation - drug effects; Ceramics; Chemistry and Materials Science; Coagulation; Composites; Dextrans - administration & dosage; Dextrans - chemistry; Dextrans - metabolism; Dextrans - pharmacology; Embolization, Therapeutic - methods; Gelatin Sponge, Absorbable - chemical synthesis; Gelatin Sponge, Absorbable - chemistry; Gelatin Sponge, Absorbable - metabolism; Glass; Hemorrhage - therapy; Humans; Hydrogels; Hydrogels - administration & dosage; Hydrogels - chemical synthesis; Hydrogels - chemistry; Hydrogels - pharmacology; Injections, Intralesional; Materials Science; Materials Testing; Molecular weight; Natural Materials; Oxidation-Reduction; Phase Transition; Polymer Sciences; Polymers; Regenerative Medicine/Tissue Engineering; Surfaces and Interfaces; Thin Films; Thrombelastography; Time Factors; Dextran; Fibrin Sealant; Chitosan Derivative; Chitosan; Gelation Time
• ISSN: 0957-4530; 1573-4838
• DOI: 10.1007/s10856-009-3721-5

We report the preparation of in situ-forming hydrogels, composed of oxidized dextran (Odex) and amine-containing polymers, for their potential use as a wound dressing to promote blood clotting. Dextran was oxidized by sodium periodate to introduce aldehyde groups to form hydrogels, upon mixing in solution with different polymers containing primary amine groups, including polyallylamine (PAA), oligochitosan and glycol chitosan. A series of experiments were conducted to identify the optimum gelation condition for the Odex-PAA system. The polymer concentration appeared to have a major effect on gelation time and the polymer weight ratio affected the resulting gel content and swelling. Other influencing factors included pH of the buffer used to dissolve each polymer, PAA molecular weight, and the type of individual material. The latter also contributed significantly to gel content and swelling. Thromboelastography was used to examine the effects of the in situ gelation on blood coagulation in vitro, where the Odex-PAA combination was found to be most pro-hemostatic, as indicated by a decrease in clotting time and an increase in clot strength. The results of this study demonstrated that in situ-forming hydrogels could promote clotting in vitro; however, further studies are required to determine if the same hydrogel formulations are effective in controlling hemorrhage in vivo .