A europium- and terbium-coated magnetic nanocomposite as sorbent in dispersive solid phase extraction coupled with ultra-high performance liquid chromatography for antibiotic determination in meat samples

• Published in: Journal of Chromatography A Vol. 1425; pp. 73 - 80
• Main Authors: Castillo-García, M.L., Aguilar-Caballos, M.P., Gómez-Hens, A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 18.12.2015
• Subjects: Animals; Anti-Bacterial Agents - analysis; Chickens; Chromatography, High Pressure Liquid - methods; Drug Residues - analysis; Eu- and Tb-coated magnetic nanoparticles; Europium - chemistry; Fluorometric detection; Meat - analysis; Meat samples; Nanocomposites; Quinolones; Quinolones - analysis; Solid Phase Extraction - methods; Swine; Terbium - chemistry; Tetracyclines; Tetracyclines - analysis; Ultra-performance liquid chromatography; Veterinary Drugs - analysis; Meat samples; Eu- and Tb-coated magnetic nanoparticles; Fluorometric detection; Tetracyclines; Ultra-performance liquid chromatography; Quinolones
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2015.11.048

•A new Eu- and Tb-coated magnetic nanomaterial (NM) has been synthesized.•This NM has been used in dispersive SPE of tetracyclines (TCs) and quinolones (Qs).•A new UHPLC separation for six TCs and Qs in less than 5min is reported.•The method application to the analysis of meat samples has yielded good results.•The low organic solvent consumption ensures an environmentally friendly procedure. A new magnetic dispersive solid-phase extraction approach based on Eu- and Tb-coated magnetic nanocomposites, combined with ultra-high performance liquid chromatography with fluorometric detection, is reported for the extraction and simultaneous determination of veterinary antibiotics. The method is aimed at monitoring of potential residues of three tetracyclines, namely oxytetracycline, tetracycline, chlortetracycline and three acidic quinolones, such as oxolinic acid, nalidixic acid and flumequine, chosen as model analytes, in animal muscle samples. The nanocomposites were obtained by synthesizing magnetic nanoparticles by a co-precipitation method and their coating with terbium and europium ions. The limits of detection obtained using standard solutions were: 1.0, 1.5, 3.8, 0.25, 0.7 and 1.2ngmL−1, which corresponds to 3.3, 5.0, 12.7, 0.8, 2.3 and 4.0μgkg−1 for oxytetracycline, tetracycline, chlortetracycline, oxolinic acid, nalidixic acid and flumequine, respectively, in meat samples. The precision values, obtained in the presence of the sample matrix, were in the ranges 0.12–2.0% and 2.6–15.4% for retention times and areas, respectively. The selectivity of the method was checked by assaying different veterinary drugs, finding that most of them did not interfere at the same concentration levels as that of analytes. A recovery study was performed in the presence of chicken and pork muscle samples, which provided values in the range of 61.5–102.6%.