Developmental and hypoxia-induced cell death share common ultrastructural and biochemical apoptotic features in the central nervous system

• Published in: Neuroscience Vol. 252; pp. 190 - 200
• Main Authors: Pozo Devoto, V.M, Bogetti, M.E, Fiszer de Plazas, S
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Ltd 12.11.2013; Elsevier
• Subjects: Animals; apoptosis; Apoptosis - physiology; Biological and medical sciences; Blotting, Western; Chick Embryo; chick optic tectum; Chickens; development; Eye and associated structures. Visual pathways and centers. Vision; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; hypoxia; Hypoxia - pathology; In Situ Nick-End Labeling; Microscopy, Electron, Transmission; Neurology; neuronal death; Superior Colliculi - embryology; Superior Colliculi - metabolism; Superior Colliculi - pathology; Vertebrates: nervous system and sense organs; HI; naturally occurring cell death; stratum griseum superficiale; hypoxic cell death; NOCD; phosphate buffer; apoptosis; phosphate-buffered saline; TdT-mediated dUTP nick-end labeling; TUNEL; optic tectum; ethylenediaminetetraacetic acid; embryonic day; hypoxic ischemic; sodium dodecyl sulfate; retinal ganglion cells; 3,3′-diaminobenzidine tetrahydrochloride; OL; optic lobe; ED; Triton X-100; PBS; hypoxia; development; SDS; DAB; C’h-i-j; OT; chick optic tectum; EDTA; TX-100; PVDF; SGC; RGC; PB; HxCD; neuronal death; polyvinylidene fluoride; cell compartment ‘h-i-j’; Oxygen; Central nervous system; Environmental factor; Optic tectum; Encephalon; Vertebrata; Neuron; Visual pathway; Cell death; Ultrastructure; Animal; Development; Hypoxia; Chicken; Aves; Apoptosis
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2013.07.065

Highlights • Ultrastructural features of HxCD during development are indistinguishable from those of NOCD. • The apoptotic intrinsic pathway is activated in hypoxic and developmental cell death. • Retinal afferent fibers do not regulate OT neuronal death on ED12. • The degeneration of neuronal processes has unique ultrastructural features.