CREB promotes laryngeal cancer cell migration via MYCT1/NAT10 axis

CREB, MYCY1 and NAT10 are involved in cancer cell migration. However, the relationship between these three proteins and their role in laryngeal cancer cell migration remains unknown. Transient gene transfection was performed in laryngeal cancer cells. Bioinformatics analysis was used to predict the...

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Published inOncoTargets and therapy Vol. 11; pp. 1323 - 1331
Main Authors Zhang, Zhao-Xiong, Zhang, Wan-Ni, Sun, Yuan-Yuan, Li, Yun-Hui, Xu, Zhen-Ming, Fu, Wei-Neng
Format Journal Article
LanguageEnglish
Published New Zealand Taylor & Francis Ltd 2018
Dove Medical Press
Subjects
Binding sites
Bioinformatics
Biotechnology industry
Cell adhesion & migration
Colorectal cancer
CREB
Gene expression
Immunoglobulins
Laryngeal cancer
Liver cancer
Medical prognosis
Metastasis
MicroRNAs
migration
MYCT1
NAT10
Original Research
Otolaryngology
Ovarian cancer
Proteins
Tumorigenesis
Variance analysis
United States > US
China
migration
NAT10
MYCT1
laryngeal cancer
CREB
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Summary:CREB, MYCY1 and NAT10 are involved in cancer cell migration. However, the relationship between these three proteins and their role in laryngeal cancer cell migration remains unknown. Transient gene transfection was performed in laryngeal cancer cells. Bioinformatics analysis was used to predict the binding of CREB to MYCT1 promoter. Binding of CREB to the promoter of MYCT1 was monitored by luciferase reporter assay and chromatin immuno-precipitation method in vitro and in vivo, respectively. Real-time RT-PCR and Western bolt were applied to detect gene transcription and translation levels, respectively. Laryngeal cancer cell migration was assayed by transwell chamber experiment. CREB protein expression was significantly up-regulated in laryngeal cancer tissues and associated with cancer differentiation, tumor stage, and lymphatic metastasis. CREB inhibits MYCT1 expression by direct binding to its promoter. Meanwhile, MYCT1 has a negative impact on the NAT10 gene expression. Furthermore, CREB promotes NAT10 expression via down-regulating the MYCT1 gene expression. In addition, contrary to MYCT1, CREB and NAT10 enhanced laryngeal cancer cell migration. MYCT1 and NAT10 significantly rescued the effects of CREB and MYCT1 on Hep2 cell migration, respectively. CREB promotes laryngeal cancer cell migration via MYCT1/NAT10 axis, suggesting that CREB might be a potential prognostic marker in laryngeal cancer.
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ISSN:1178-6930
1178-6930
DOI:10.2147/OTT.S156582