Rb analog Whi5 regulates G1 to S transition and cell size but not replicative lifespan in budding yeast

• Published in: Translational medicine of aging Vol. 3; pp. 104 - 108
• Main Authors: Crane, Matthew M., Tsuchiya, Mitsuhiro, Blue, Ben W., Almazan, Jared D., Chen, Kenneth L., Duffy, Siobhan R., Golubeva, Alexandra, Grimm, Annaiz M., Guard, Alison M., Hill, Shauna A., Huynh, Ellen, Kelly, Ryan M., Kiflezghi, Michael, Kim, Hyunsung D., Lee, Mitchell, Lee, Ting-I., Li, Jiayi, Nguyen, Bao M.G., Whalen, Riley M., Yeh, Feng Y., McCormick, Mark, Kennedy, Brian K., Delaney, Joe R., Kaeberlein, Matt
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 2019; KeAi Communications Co., Ltd
• Subjects: Cell cycle regulation; Replicative aging; Saccharomyces cerevisiae; Replicative aging; Cell cycle regulation; Saccharomyces cerevisiae
• ISSN: 2468-5011; 2468-5011
• DOI: 10.1016/j.tma.2019.10.002

An increase in cell size with age is a characteristic feature of replicative aging in budding yeast. Deletion of the gene encoding Whi5 results in shortened duration of G1 and reduced cell size, and has been previously suggested to increase replicative lifespan. Upon careful analysis of multiple independently derived haploid and homozygous diploid whi5Δ mutants, we find no effect on lifespan, but we do confirm the reduction in cell size. We suggest that instead of antagonizing lifespan, the elongated G1 phase of the cell cycle during aging may actually play an important role in allowing aged cells time to repair accumulating DNA damage.