Mu opioid receptor mRNA expression, binding, and functional coupling to G-proteins in human epileptic hippocampus
Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lob...
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Published in | Hippocampus Vol. 22; no. 2; pp. 122 - 127 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
01.02.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Mu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast with autopsy samples, hippocampus obtained from patients with mesial TLE demonstrated enhanced MOR mRNA expression (116%). Saturation binding experiments revealed significantly higher (60%) Bmax values for the mesial TLE group, whereas the Kd values were not statistically different. Although mesial TLE group demonstrated high levels of basal binding for the G proteins (136%), DAMGO‐stimulated [35S]GTPγS binding did not demonstrate significant alterations. In conclusion, our present data provide strong evidence that the epileptic hippocampus of patients with pharmacoresistant mesial TLE presents significant alterations in MOR. Such changes may represent adaptive mechanisms to compensate for other as yet unknown alterations. © 2010 Wiley Periodicals, Inc. |
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Bibliography: | National Institute of Science and Technology of Distrito Federal, Academy of Sciences of Hungary - No. ETT577/2006; No. RET67/2005 Hungarian-Turkish project 11-06 National Council for Sciences and Technology of Mexico - No. J110.0130/2009; No. 98386 ArticleID:HIPO20891 Technological and Scientific Council of Turkey (TUBITAK) - No. 106S249 istex:8163C10A5D2A431758365EA6DCBBABE87701F527 ark:/67375/WNG-VNXVZZRS-2 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1050-9631 1098-1063 |
DOI: | 10.1002/hipo.20891 |