Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose

• Published in: Talanta (Oxford) Vol. 120; pp. 362 - 367
• Main Authors: Zhang, Weimin, Ma, Diao, Du, Jianxiu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.03.2014
• Subjects: Biomimetic Materials - chemistry; Biomimetics - methods; Biosensing Techniques - methods; Blood Glucose - analysis; Catalysis; Colorimetric; Colorimetry; Colorimetry - methods; Coloring Agents - chemistry; Ferrocyanides - chemistry; Glucose; Humans; Hydrogen peroxide; Hydrogen Peroxide - analysis; Limit of Detection; Nanoparticles; Nanoparticles - chemistry; Nanostructure; Oxidation; Peroxidase; Peroxidase - chemistry; Peroxidase-like activity; Prussian blue nanoparticles; Prussian blue nanoparticles; Peroxidase-like activity; Hydrogen peroxide; Glucose; Colorimetric
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2013.12.028

Prussian blue nanoparticles (PB NPs) exhibits an intrinsic peroxidase-like catalytic activity towards the hydrogen peroxide (H2O2)-mediated oxidation of classical peroxidase substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt to produce a colored product. The catalysis follows Michaelis–Menen kinetics and shows strong affinity for H2O2. Using PB NPs as a peroxidase mimetics, a colorimetric method was developed for the detection of 0.05–50.0μM H2O2, with a detection limit of 0.031μM. When the catalytic reaction of PB NPs was coupled with the reaction of glucose oxidation catalyzed by glucose oxidase, a sensitive and selective colorimetric method for the detection of glucose was realized. The limit of detection for glucose was determined to be as low as 0.03μM and the linear range was from 0.1μM to 50.0μM. The method was successfully applied to the determination of glucose in human serum. Compared with other nanomaterials-based peroxidase mimetics, PB NPs provides 10–100 times higher sensitivity toward the detection of H2O2 and glucose. The detection platform developed showed great potential applications in varieties of physiological importance substances when merged with appropriate H2O2-producing oxidases. Prussian blue nanoparticles exhibit intrinsic peroxidase-like activity, providing a highly sensitive colorimetric detection method for H2O2 and glucose. [Display omitted] •Prussian blue nanoparticles were demonstrated to exhibits intrinsic peroxidase-like catalytic activity.•Prussian blue nanoparticles was successfully used as a peroxidase mimetics for highly sensitive colorimetric detection of H2O2 and glucose.•The method can be applied to the determination of glucose in human serum samples.