Analysis of the individual allergens of Russian thistle pollen by an enzyme-linked immunoblotting technique

Russian thistle pollen extract was analyzed by immunoblots of isoelectric focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Sev...

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Bibliographic Details
Published inThe Journal of asthma Vol. 26; no. 4; p. 243
Main Authors Gomez, J, Mansfield, L E, Frederick, R W, Rael, E D
Format Journal Article
LanguageEnglish
Published England 1989
Subjects
Allergens - analysis
Allergens - chemistry
Electrophoresis, Polyacrylamide Gel
Humans
Immunoblotting - methods
Isoelectric Focusing
Molecular Weight
Pollen
Skin Tests
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Summary:Russian thistle pollen extract was analyzed by immunoblots of isoelectric focused and SDS-PAGE gels. Twenty distinct protein bands were recognized by human IgE- and IgG-specific antibodies in the immunoblot from the SDS-PAGE gel. Molecular weights of these allergens ranged from 12.2 kD to 85 kD. Seventeen bands were detected on isoelectric focusing immunoblots with pI from 3.95 to 7.70. Allergic subjects had differing individual patterns of protein band recognition. Immunoblot techniques provide detailed evaluations of the response of allergy subjects to components of crude natural allergens.
ISSN:0277-0903
1532-4303
DOI:10.3109/02770908909073256