On-site enzymes produced from Trichoderma reesei RUT-C30 and Aspergillus saccharolyticus for hydrolysis of wet exploded corn stover and loblolly pine

• Published in: Bioresource technology Vol. 154; pp. 282 - 289
• Main Authors: Rana, Vandana, Eckard, Anahita D., Teller, Philip, Ahring, Birgitte K.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.02.2014; Elsevier
• Subjects: A. saccharolyticus; Agronomy. Soil science and plant productions; Aspergillus - enzymology; Biological and medical sciences; Cellulase; Cellulase - metabolism; Cellulase production; Corn; Enzymes; Feedstock; Food industries; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; Hydrogen-Ion Concentration; Hydrolysis; Oxygen - analysis; Pine; Pinus taeda - drug effects; Pinus taeda - metabolism; Sugars; T. reesei RUT-C30; Temperature; Trichoderma - enzymology; Trichoderma reesei; Use and upgrading of agricultural and food by-products. Biotechnology; Use of agricultural and forest wastes. Biomass use, bioconversion; Water - pharmacology; Wet explosion; Zea mays - drug effects; Zea mays - metabolism; β-Glucosidase; β-Glucosidase; Wet explosion; A. saccharolyticus; Cellulase production; T. reesei RUT-C30; Monocotyledones; In situ; Pinus taeda; Cereal crop; Straw; Fungi; Aspergillus; Gramineae; Angiospermae; Softwood forest tree; Gymnospermae; Fungi Imperfecti; Zea mays; Enzyme; Explosions; Trichoderma reesei; Glycosylases; Hydrolysis; Cellulolytic enzyme; Cellulase; Glycosidases; Hydrolases; Coniferales; Spermatophyta; Corn by product
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.12.059

Cellulase production by two filamentous fungi Trichoderma reesei RUT-C30 and novel fungal strain, Aspergillus saccharolyticus on pretreated corn stover was investigated. Cellulase production was followed by the hydrolysis of two feedstocks, wet-exploded corn stover (WECS) and wet-exploded loblolly pine (WELP) by on-site produced enzyme cocktails containing cellulase from T. reesei RUT-C30 and β-glucosidase from A. saccharolyticus. The sugar yields using the on-site enzyme cocktails were compared with commercial enzymes preparations, Celluclast 1.5L and Novozym 188 at two substrate concentrations, 5% and 10% (w/w) and enzyme loading at 5 and 15FPU/g glucan for WECS and WELP. The highest sugar yields were obtained at 5% (w/w) substrate concentration and 15FPU/g glucan for both feedstocks. Glucose yields of 81% and 88% were obtained from on-site and commercial enzymes, respectively using WECS as feed stock. The sugar yields were 55% and 58% for WELP samples hydrolyzed with on-site and commercial enzymes, respectively. •We report on-site enzymes production from fungi using side-stream of biorefinery.•On-site enzymes showed high hydrolytic efficiency and sugar yields on WEx biomass.•Sugars yields by on-site produced enzymes and commercial enzymes were comparable.