Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas

• Published in: Science China. Life sciences Vol. 57; no. 3; pp. 315 - 326
• Main Authors: Zeng, ZhaoYang, Huang, HongBin, Huang, LiLi, Sun, MengXi, Yan, QiJia, Song, YaLi, Wei, Fang, Bo, Hao, Gong, ZhaoJian, Zeng, Yong, Li, Qiao, Zhang, WenLing, Li, XiaYu, Xiang, Bo, Li, XiaoLing, Li, Yong, Xiong, Wei, Li, GuiYuan
• Format: Journal Article
• Language: English
• Published: Beijing Science China Press 01.03.2014; Springer Nature B.V
• Subjects: 3' Untranslated Regions - genetics; Animals; beta-Transducin Repeat-Containing Proteins - genetics; beta-Transducin Repeat-Containing Proteins - metabolism; Biomedical and Life Sciences; Blotting, Western; Cell Line, Tumor; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - metabolism; EB病毒; Epithelium - metabolism; Epstein-Barr virus; Epstein-Barr病毒; Gene Expression Regulation, Neoplastic; Gene Expression Regulation, Viral; Gene Regulatory Networks; HEK293 Cells; Herpesvirus 4, Human - genetics; Herpesvirus 4, Human - physiology; Host-Pathogen Interactions - genetics; Humans; Life Sciences; microRNA; MicroRNAs - genetics; miRNA; Nasopharyngeal Neoplasms - genetics; Nasopharyngeal Neoplasms - metabolism; Nasopharyngeal Neoplasms - virology; Nasopharynx - metabolism; Nasopharynx - pathology; Research Paper; Reverse Transcriptase Polymerase Chain Reaction; RNA, Viral - genetics; Transcriptome; 主基因; 基因调控网络; 表达谱; 鼻咽癌; nasopharyngeal carcinoma (NPC); microRNA (miRNA); Epstein-Barr virus (EBV); gene regulate network
• ISSN: 1674-7305; 1869-1889
• DOI: 10.1007/s11427-013-4577-y

Epstein-Barr virus （EBV） is associated with nasopharyngeal carcinoma （NPC） tumorigenesis. However, the mechanism（s） connecting EBV infection and NPC remain unclear. Recently, a new class of EBV microRNAs （miRNAs） has been described. To determine how EBV miRNAs control the expression of host genes, and to understand their potential role in NPC tumor- igenesis, we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyn- geal epithelial tissues. We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC. Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the car- cinogenesis of NPC. A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated, sug- gesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis. An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis.