Graphene oxide as nanocarrier for sensitive electrochemical immunoassay of clenbuterol based on labeling amplification strategy

• Published in: Talanta (Oxford) Vol. 107; pp. 176 - 182
• Main Authors: Lai, Yanjun, Bai, Jing, Shi, XinHao, Zeng, Yanbo, Xian, Yuezhong, Hou, Jie, Jin, Litong
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.03.2013
• Subjects: Adrenergic beta-Agonists - analysis; Animal Feed - analysis; Animals; Aspergillus niger - enzymology; Clenbuterol; Clenbuterol - analysis; Electrochemical immunosensor; Electrochemical Techniques - methods; Enzymes, Immobilized - chemistry; Glucose Oxidase - chemistry; Glucose oxide; Gold - chemistry; Gold nanoparticles; Graphene oxide; Graphite - chemistry; Immunoassay - methods; Limit of Detection; Nanoparticles - chemistry; Oxides - chemistry; Prussian blue; Swine; Electrochemical immunosensor; Glucose oxide; Clenbuterol; Graphene oxide; Prussian blue; Gold nanoparticles
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2013.01.002

A novel electrochemical immunosensor for sensitive detection of clenbuterol (CLB) is fabricated using glucose oxidase (GOD)-functionalized grahene oxide (GO) nanocomposites to label CLB. The immunosensor was constructed by layer-by-layer assembly colloidal prussian blue (PB), multiwalled carbon nanotubes (MWCNTs) and CLB antibodies (Abs) on a glassy carbon electrode (GCE). In this competitive immunoassay system, PB acts as the redox mediator to reduce H2O2 originated from the catalyst cycle of GOD. The high ratio of GOD to GO effectively amplified the signal for this competitive-type immunoassay. Under optimized conditions, the immunosensor shows a wide linear range from 0.5 to 1000ng/mL with a low detection limit of 0.25ng/mL. The dual signal amplification of GOD-functionalized GO nanocomposites as a label is promising to be applied to design other sensitive immunosenseors. ► Labeling amplification strategy was used to construct the clenbuterol immunosensor. ► Gold nanoparticles decorated graphene oxide was utilized as a nanocarrier. ► The circles directed by glucose oxide and Prussian blue dually amplified signal. ► The proposed immunosensor achieves high sensitivity and a low detection limit.