Single Cell Multiomics Identifies Cells and Genetic Networks Underlying Alveolar Capillary Dysplasia

• Published in: American journal of respiratory and critical care medicine Vol. 208; no. 6; pp. 709 - 725
• Main Authors: Guo, Minzhe, Wikenheiser-Brokamp, Kathryn A., Kitzmiller, Joseph A., Jiang, Cheng, Wang, Guolun, Wang, Allen, Preissl, Sebastian, Hou, Xiaomeng, Buchanan, Justin, Karolak, Justyna A., Miao, Yifei, Frank, David B., Zacharias, William J., Sun, Xin, Xu, Yan, Gu, Mingxia, Stankiewicz, Pawel, Kalinichenko, Vladimir V., Wambach, Jennifer A., Whitsett, Jeffrey A.
• Format: Journal Article
• Language: English
• Published: United States American Thoracic Society 15.09.2023
• Subjects: Cellular biology; Endothelial Cells - pathology; Forkhead Transcription Factors - genetics; Gene Regulatory Networks - genetics; Genes; Humans; Infant, Newborn; Lung - pathology; Lung diseases; Medical research; Multiomics; Original; Persistent Fetal Circulation Syndrome - genetics; Persistent Fetal Circulation Syndrome - pathology; Ribonucleic acid; RNA; Vascular Endothelial Growth Factor A - genetics; alveolar capillary dysplasia; FOXF1; pulmonary microvasculature
• ISSN: 1073-449X; 1535-4970; 1535-4970
• DOI: 10.1164/rccm.202210-2015OC

Alveolar capillary dysplasia with misalignment of pulmonary veins (ACDMPV) is a lethal developmental disorder of lung morphogenesis caused by insufficiency of FOXF1 (forkhead box F1) transcription factor function. The cellular and transcriptional mechanisms by which FOXF1 deficiency disrupts human lung formation are unknown. To identify cell types, gene networks, and cell-cell interactions underlying the pathogenesis of ACDMPV. We used single-nucleus RNA and assay for transposase-accessible chromatin sequencing, immunofluorescence confocal microscopy, and RNA hybridization to identify cell types and molecular networks influenced by in ACDMPV lungs. Pathogenic single-nucleotide variants and copy-number variant deletions involving the gene locus in all subjects with ACDMPV (  = 6) were accompanied by marked changes in lung structure, including deficient alveolar development and a paucity of pulmonary microvasculature. Single-nucleus RNA and assay for transposase-accessible chromatin sequencing identified alterations in cell number and gene expression in endothelial cells (ECs), pericytes, fibroblasts, and epithelial cells in ACDMPV lungs. Distinct cell-autonomous roles for in capillary ECs and pericytes were identified. Pathogenic variants involving the gene locus disrupt gene expression in EC progenitors, inhibiting the differentiation or survival of capillary 2 ECs and cell-cell interactions necessary for both pulmonary vasculogenesis and alveolar type 1 cell differentiation. Loss of the pulmonary microvasculature was associated with increased VEGFA (vascular endothelial growth factor A) signaling and marked expansion of systemic bronchial ECs expressing COL15A1 (collagen type XV α 1 chain). Distinct gene regulatory networks were identified in subsets of pulmonary endothelial and fibroblast progenitors, providing both cellular and molecular targets for the development of therapies for ACDMPV and other diffuse lung diseases of infancy.