Nanopore Sequencing Technology as an Emerging Tool for Diversity Studies of Plant Organellar Genomes

In this comprehensive review, we explore the significant role that nanopore sequencing technology plays in the study of plant organellar genomes, particularly mitochondrial and chloroplast DNA. To date, the application of nanopore sequencing has led to the successful sequencing of over 100 plant mit...

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Bibliographic Details
Published inDiversity (Basel) Vol. 16; no. 3; p. 173
Main Authors Sawicki, Jakub, Krawczyk, Katarzyna, Paukszto, Łukasz, Maździarz, Mateusz, Kurzyński, Mateusz, Szablińska-Piernik, Joanna, Szczecińska, Monika
Format Journal Article
LanguageEnglish
Published Basel MDPI AG 01.03.2024
Subjects
Accuracy
adaptive sampling
Algorithms
Bar codes
Chloroplast DNA
Chloroplasts
Complexity
Data processing
Deoxyribonucleic acid
DNA
DNA sequencing
Gene sequencing
Genes
Genetic research
Genetic testing
Genomes
Genomics
Methods
Mitochondrial DNA
Molecular biology
nanopore sequencing
Nucleotide sequence
Nucleotide sequencing
Plant genetics
plant mitogenome
plastome
target enrichment
Trends
Poland
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Summary:In this comprehensive review, we explore the significant role that nanopore sequencing technology plays in the study of plant organellar genomes, particularly mitochondrial and chloroplast DNA. To date, the application of nanopore sequencing has led to the successful sequencing of over 100 plant mitochondrial genomes and around 80 chloroplast genomes. These figures not only demonstrate the technology’s robustness but also mark a substantial advancement in the field, highlighting its efficacy in decoding the complex and dynamic nature of these genomes. Nanopore sequencing, known for its long-read capabilities, significantly surpasses traditional sequencing techniques, especially in addressing challenges like structural complexity and sequence repetitiveness in organellar DNA. This review delves into the nuances of nanopore sequencing, elaborating on its benefits compared to conventional methods and the groundbreaking applications it has fostered in plant organellar genomics. While its transformative impact is clear, the technology’s limitations, including error rates and computational requirements, are discussed, alongside potential solutions and prospects for technological refinement.
ISSN:1424-2818
1424-2818
DOI:10.3390/d16030173