High performance liquid chromatographic determination of EDTA, calcium (II)-EDTA and iron (III)-EDTA in canned and bottled foods

• Published in: Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 26; no. 3; pp. 253 - 259_1
• Main Authors: Yamaguchi, A. (Sapporo-shi. Inst. of Public Health (Japan)), Yamaguchi, T, Shiroishi, Y, Shimizu, Y, Takasugi, N
• Format: Journal Article
• Language: English; Japanese
• Published: Japanese Society for Food Hygiene and Safety 01.01.1985
• Subjects: ANALISIS QUIMICO; ANALYSE CHIMIQUE; ANTIOXIDANTES; ANTIOXIDANTS; ANTIOXYDANT; bottled food; BOTTLED PRODUCTS; CALCIO; CALCIUM; calcium (II) ethylenediaminetetraacetate; canned food; CANNED FOODS; CHELATEUR; CHELATING AGENTS; CHEMICAL ANALYSIS; CHROMATOGRAPHIE LIQUIDE HAUTE PRESS; CONSERVAS ALIMENTICIAS; CONSERVE ALIMENTAIRE; CROMATOGRAFIA LIQUIDA ALTA PRESION; ethylenediaminetetraacetate (EDTA); FER; HIERRO; high performance liquid chromatography; HIGH PRESSURE LIQUID CHROMATOGRAPHY; IRON; iron (III) ethylenediaminetetraacetate; PRODUCTOS EMBOTELLADOS; PRODUIT EMBOUTEILLE; QUELATOS
• ISSN: 0015-6426; 1882-1006
• DOI: 10.3358/shokueishi.26.253

A practical method was developed for the fractional determination of ethylenediaminetetraacetate (EDTA), Ca (II)-EDTA and Fe (III)-EDTA in canned and bottled foods. Bismuth (III) subnitrate suspended in sodium chloride-ammonium buffer solution was suitable for conversion of EDTA to the equivalent amount of Bi (III)-EDTA at pH 10, leaving Ca (II)- and Fe (III)-EDTA intact. Further, Ca (II)-EDTA was converted to Cu (II)-EDTA in the presence of Cu (II) ion at pH 2-3, leaving Bi (III)- and Fe (III)-EDTA unaltered. The resulting EDTA-chelates of Bi (III), Cu (II) and Fe (III) were separated by high performance liquid chromatography on a Nucleosil 5C18 column using 2M ammonium sulfate solution as the eluent. The recoveries of EDTA, Ca (II)-EDTA and Fe (III)-EDTA were satisfactory. It was found that the addition of EDTA to foods caused the formation of Ca (II)-EDTA in all samples and that of Fe (III)-EDTA in several samples. Four imported food samples were analyzed by the proposed method. One sample, sandwich spread, contained 29.2μg/g of Ca (II)-EDTA and 11.0μg/g of Fe (III)-EDTA.