Endoplasmic reticulum as a site of phenylpropanoid and flavonoid metabolism in Hippeastrum

The nature of bound forms of enzymes of phenylpropanoid and flavonoid metabolism have been investigated in Hippeastrum CV Dutch Red Hybrid. Particulate compenents of petal homogenates were fractionated on sucrose gradients and the EDTA shift method was employed to characterize membranes of the endop...

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Published inPlant physiology (Bethesda) Vol. 74; no. 4; pp. 901 - 906
Main Authors Wagner, George J., Hrazdina, Geza
Format Journal Article
LanguageEnglish
Published United States American Society of Plant Physiologists 01.04.1984
Subjects
Biosynthesis
Cytoplasm
Cytosol
endoplasmic reticulum
Enzymes
Flavonoids
Hippeastrum
Metabolism
Particulate matter
Petals
Protoplasts
Vacuoles
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Summary:The nature of bound forms of enzymes of phenylpropanoid and flavonoid metabolism have been investigated in Hippeastrum CV Dutch Red Hybrid. Particulate compenents of petal homogenates were fractionated on sucrose gradients and the EDTA shift method was employed to characterize membranes of the endoplasmic reticulum. In magnesium-containing gradients, a portion of phenylalanine ammonia lyase, chalcone synthase, glucosyl transferase, and all of the trans-cinnamate 4-monooxygenase and NADH Cytochrome c reductase (the last an endoplasmic reticulum marker) were associated with membranes equilibrating at 1.18 specific gravity. In gradients lacking magnesium and containing EDTA, the above activities--except chalcone synthase, which was lost--and protein were diminished at 1.18 specific gravity and enhanced at lower densities characteristic of membranes of the smooth endoplasmic reticulum. These results are consistent with the contention that endoplasmic reticulum is a site of phenylpropanoid and flavonoid metabolism in Hippeastrum.
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ISSN:0032-0889
1532-2548
DOI:10.1104/pp.74.4.901