Molecular cloning of GA-suppressed G2 pea genes by cDNA RDA

• Published in: Science China. Life sciences Vol. 40; no. 4; pp. 379 - 383
• Main Author: 朱玉贤 张翼凤 李慧英
• Format: Journal Article
• Language: English
• Published: China Springer Nature B.V 01.08.1997
• Subjects: Cloning; Differential display; GA-suppressed; Gene expression; genes; Hybridization; molecular; pea; Pisum sativum; RDA
• ISSN: 1674-7305; 1006-9305; 1869-1889; 1862-2798
• DOI: 10.1007/BF02881731

GA-treated and non-treated G2 pea cDNAs were compared using a newly developed method called cDNA representational difference analysis (cDNA-RDA), and several GA-suppressed mRNAs were found. After cloning of the larger fragments PGAS1-3 ( pea GA-suppressed cDNA 1-3), they were demonstrated to be expr...