Molecular cloning of GA-suppressed G2 pea genes by cDNA RDA

GA-treated and non-treated G2 pea cDNAs were compared using a newly developed method called cDNA representational difference analysis (cDNA-RDA), and several GA-suppressed mRNAs were found. After cloning of the larger fragments PGAS1-3 ( pea GA-suppressed cDNA 1-3), they were demonstrated to be expr...

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Bibliographic Details
Published inScience China. Life sciences Vol. 40; no. 4; pp. 379 - 383
Main Author 朱玉贤 张翼凤 李慧英
Format Journal Article
LanguageEnglish
Published China Springer Nature B.V 01.08.1997
Subjects
Cloning
Differential display
GA-suppressed
Gene expression
genes
Hybridization
molecular
pea
Pisum sativum
RDA
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Summary:GA-treated and non-treated G2 pea cDNAs were compared using a newly developed method called cDNA representational difference analysis (cDNA-RDA), and several GA-suppressed mRNAs were found. After cloning of the larger fragments PGAS1-3 ( pea GA-suppressed cDNA 1-3), they were demonstrated to be expressed only in pea tissue not treated with GA3 through Northern analysis. Compared with subtractive hybridization and differ-ential display techniques, this method not only can be easily manipulated but also has a relatively low rate of false posi-tive and is highly repetitive. It is the major progress in molecular cloning techniques.
Bibliography:11-5841/Q
ZHU Yuxian , ZHANG Yifeng and LI Huiying(State Key Laboratory of Protein Engineering and Plant Genetic Engineering, Beijing 100871, China)
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ISSN:1674-7305
1006-9305
1869-1889
1862-2798
DOI:10.1007/BF02881731