Deletion of Smad3 prevents renal fibrosis and inflammation in type 2 diabetic nephropathy

• Published in: Metabolism, clinical and experimental Vol. 103; p. 154013
• Main Authors: Xu, Bi-Hua, Sheng, Jingyi, You, Yong-Ke, Huang, Xiao-Ru, Ma, Ronald C.W., Wang, Qingwen, Lan, Hui-Yao
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.02.2020
• Subjects: Albuminuria - complications; Albuminuria - genetics; Animals; Diabetes Mellitus, Experimental - complications; Diabetes Mellitus, Experimental - genetics; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - genetics; Diabetic Nephropathies - genetics; Diabetic Nephropathies - prevention & control; Diabetic nephropathy; Female; Fibrosis; Fibrosis - genetics; Fibrosis - prevention & control; Gene Deletion; Inflammation; Kidney - pathology; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Smad3 and TGF-β; Smad3 Protein - genetics; EndoMT; T1DN; MCP-1; PAS; IHC; JNK; LncRNA; DN; ICAM-1; T2DN; CTGF; SE; GBM; ANOVA; Col-IV; STZ; P-NF-κB/65; TGF-β; Diabetic nephropathy; AngII; WT; AGE; DAB; KO; VEGF; Inflammation; IL-8; MAPK; EMT; IL-6; PASM; UUO; ACR; P-IκBα; SIS3; Smad3 and TGF-β; TNF-α; Fibrosis; Col-I; P-Smad3; PCR; Erk
• ISSN: 0026-0495; 1532-8600
• DOI: 10.1016/j.metabol.2019.154013

Transforming growth factor (TGF)-β/Smad3 signaling is highly activated in kidneys of patients with type 2 diabetic nephropathy (T2DN), however, the precise role of Smad3 in the pathogenesis of diabetic nephropathy remains unclear. Smad3 knockout (KO)-db/db mice were generated by intercrossing of male and female double-heterozygous Smad3+/− db/m mice. Renal functions including urinary albumin excretion and serum creatinine were determined. Renal histological injury including renal fibrosis and inflammation were examined by periodic acid Schiff (PAS), periodic acid-silver methenamine (PASM), and immunohistochemistry (IHC) staining. Smad3 knockout (KO)-db/db mice were protected from the development of diabetic kidney injury, characterized by the normal levels of urinary albumin excretion and serum creatinine without any evidence for renal fibrosis and inflammation. In contrast, Smad3 wild-type (WT) db/db and Smad3+/− db/db mice developed progressively decline in renal function over the 12 to 32-week time course, including increased microalbuminuria and elevated levels of serum creatinine. Pathologically, Smad3 WT db/db and Smad3+/− db/db mice exhibited a marked deposition of collagen-I (colI), collagen-IV(col-IV), and an increased infiltration of F4/80+ macrophages in kidney. Mechanistically, Smad3 deficiency decreased the lncRNA Erbb4-IR transcription, while increased miR-29b transcription and therefore protected the kidney from progressive renal injury in db/db mice. Results from this study imply that Smad3 may represent as a novel and effective therapeutic target for T2DN. •Smad3 deficiency prevents renal dysfunction in type 2 diabetic nephropathy.•Smad3 deficiency prevents renal fibrosis and inflammation in type 2 diabetic nephropathy.•Smad3 deficiency decreased the lncRNA Erbb4-IR transcription, while increased miR-29b transcription.