Nodule‐enhanced protease inhibitor gene: emerging patterns of gene expression in nodule development on Sesbania rostrata

• Published in: Journal of experimental botany Vol. 55; no. 394; pp. 89 - 97
• Main Authors: Lievens, Sam, Goormachtig, Sofie, Holsters, Marcelle
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 01.01.2004; Oxford Publishing Limited (England)
• Subjects: Agronomy. Soil science and plant productions; Amino Acid Sequence; Azorhizobium caulinodans - growth & development; Bacteria; Biological and medical sciences; Botrytis - growth & development; Cloning, Molecular; Complementary DNA; DNA, Complementary - chemistry; DNA, Complementary - genetics; Early nodulin; Economic plant physiology; Fabaceae - genetics; Fabaceae - growth & development; Fabaceae - microbiology; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Developmental; Gene Expression Regulation, Plant; Genes; Holsters; Infections; Inoculation; Kunitz family; Molecular Sequence Data; Mutation; Nodulation; Nodules; Parasitism and symbiosis; Peptides - genetics; Peptides - metabolism; Plant physiology and development; Plant Proteins - genetics; Plant Proteins - metabolism; Protease inhibitors; Research Papers: Regulation of Growth, Development and Whole Organism Physiology; Root primordia; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sesbania rostrata; Stress, Mechanical; Symbiosis; Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...); Symbiosis - genetics; Symbiosis - physiology; Symbiosis; Green manure; Symbiotic relation; Early nodulin; Enzyme; Biochemical marker; Plant nodule; Gene expression; Symbiont; Kunitz family; Peptidases; Inoculation; Regulation(control); Leguminosae; Dicotyledones; Angiospermae; Development; protease inhibitors; Bacteria; Hydrolases; Spermatophyta; Rhizobiaceae; Sesbania rostrata; Protease inhibitor
• ISSN: 0022-0957; 1460-2431; 1460-2431
• DOI: 10.1093/jxb/erh015

A novel marker for the early stages of nodulation of Sesbania rostrata was found to encode a putative member of the Kunitz family of protease inhibitors (SrPI1). Its expression was enhanced during nodulation, and was not up‐regulated by wounding or upon infection with wide host‐range pathogens. In situ expression patterns resembled those previously described for functions that may be implicated in delimiting infected nodule tissues from the rest of the plant. Thus, SrPI1 may be a component of a multi‐layered barrier that restrains the invading rhizobia.