Deduced Amino Acid Sequence of the Fusion Glycoprotein of Turkey Rhinotracheitis Virus has Greater Identity with that of Human Respiratory Syncytial Virus, a Pneumovirus, than that of Paramyxoviruses and Morbilliviruses

• Published in: Journal of general virology Vol. 72; no. 1; pp. 75 - 81
• Main Authors: Yu, Q, Davis, P. J, Barrett, T, Binns, M. M, Boursnell, M. E. G, Cavanagh, D
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.1991; Society for General Microbiology
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Cloning, Molecular; Fundamental and applied biological sciences. Psychology; Genetics; glycoproteins; Glycoproteins - genetics; Glycoproteins - isolation & purification; Herpesviridae - genetics; Humans; Measles virus - genetics; Microbiology; Molecular Sequence Data; Paramyxoviridae - genetics; paramyxovirus; pneumovirus; respiratory syncytial virus; Respiratory Syncytial Viruses - genetics; RNA, Viral - genetics; RNA, Viral - isolation & purification; Sequence Homology, Nucleic Acid; turkey rhinotracheitis virus; Turkeys; Viral Fusion Proteins - genetics; Viral Fusion Proteins - isolation & purification; Virology; Virus; Membrane fusion; Gene; Nucleotide sequence; Taxonomy; Glycoproteins; Molecular cloning; Comparative study; Aminoacid sequence; Paramyxoviridae
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-72-1-75

1 Institute for Animal Health, Division of Molecular Biology, Houghton Laboratory, Houghton, Huntingdon, Cambridgeshire PE17 2DA and 2 Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF, U.K. The sequence of the fusion (F) glycoprotein of turkey rhinotracheitis virus (TRTV) has been deduced from cDNA clones derived from oligo(dT)-selected infected cell RNA. The protein consists of 538 amino acids, the F2 and F1 subunits containing 102 (including the F2-F1 connecting peptide RRRR) and 436 residues, respectively. Each subunit has one potential N -linked glycosylation site. The protein has 38 to 39% amino acid identity with the F protein of respiratory syncytial virus ( Pneumovirus genus) but only about half that with members of the other two genera ( Paramyxovirus and Morbillivirus ) in the Paramyxoviridae family. This is the first sequence evidence to support the view that TRTV is a pneumovirus, the first avian member of the genus to be described. Present address: Animal Health Trust, Lanwades Park, Kennett, Newmarket, Suffolk CB8 7PN, U.K. > Present address: Immunology Limited, 184 Cambridge Science Park, Milton Road, Cambridge CB4 4GN, U.K. Received 24 August 1990; accepted 12 October 1990.