Exosomes from Adipose-Derived Mesenchymal Stem Cells Protect Against Cyclophosphamide-Induced Cardiotoxicity in Rats

A certain dosage of cyclophosphamide (CYP) in clinical applications contributes to severe cardiotoxicity. Herein, this study explored the impact of adipose-derived mesenchymal stem cell (AdMSC)-exosomes (Exos) on CYP-induced cardiotoxicity.AdMSCs and AdMSCs-Exos were isolated and identified. CYP was...

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Bibliographic Details
Published inInternational Heart Journal Vol. 64; no. 5; pp. 935 - 944
Main Authors Xiong, Jianhua, Ding, Binjun, Zhu, Wei, Xu, Lanlan, Yu, Songping
Format Journal Article
LanguageEnglish
Published Tokyo International Heart Journal Association 30.09.2023
Japan Science and Technology Agency
Subjects
Alkaline phosphatase
Apoptosis
Aspartate aminotransferase
Autophagy
Cardiac injury
Cardiotoxicity
Creatine
Creatine kinase
Cyclophosphamide
Enzyme-linked immunosorbent assay
Exosomes
Fibrosis
Heart
Immunohistochemistry
Kinases
L-Lactate dehydrogenase
Mesenchymal stem cells
Myocardial fibrosis
Oxidative stress
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Summary:A certain dosage of cyclophosphamide (CYP) in clinical applications contributes to severe cardiotoxicity. Herein, this study explored the impact of adipose-derived mesenchymal stem cell (AdMSC)-exosomes (Exos) on CYP-induced cardiotoxicity.AdMSCs and AdMSCs-Exos were isolated and identified. CYP was utilized for developing a cardiotoxicity rat model, after which blood was collected and then the serum contents of cardiac injury-related indexes (creatine kinase-MB, lactate dehydrogenase, aspartate aminotransferase, and alkaline phosphatase) were detected with enzyme-linked immunosorbent assay kits. Oxidative stress (OS)-related indicators were measured with the corresponding kits. Myocardial pathological changes and collagen fibrosis were tested with hematoxylin-eosin and Masson staining, and apoptosis-related and autophagy-related proteins in rat cardiac tissues with immunohistochemistry and Western blot assays, respectively.AdMSCs and AdMSCs-Exos were successfully isolated. AdMSCs-Exos could target rat hearts. AdMSCs-Exos improved cardiac function and diminished the content of the cardiac injury-related indexes in CYP rats. In addition, AdMSCs-Exos reduced CYP-induced cardiac fibrosis, OS, apoptosis, and autophagy in rats.AdMSCs-Exos alleviated CYP-induced cardiotoxicity in rats via the repression of OS, apoptosis, and autophagy.
ISSN:1349-2365
1349-3299
DOI:10.1536/ihj.23-201