Annona mucosa Jacq. (Annonaceae): A promising source of bioactive compounds against Sitophilus zeamais Mots. (Coleoptera: Curculionidae)

• Published in: Journal of stored products research Vol. 55; pp. 6 - 14
• Main Authors: Ribeiro, Leandro do Prado, Vendramim, José Djair, Bicalho, Keylla Utherdyany, Andrade, Moacir dos Santos, Fernandes, João Batista, Moral, Rafael de Andrade, Demétrio, Clarice Garcia Borges
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.10.2013; Elsevier
• Subjects: Acetogenins; Agronomy. Soil science and plant productions; Alkaloids; Allelochemicals; Annona; Annona mucosa; Annonaceae; Bioinsecticide; Biological and medical sciences; branches; chemical analysis; Coleoptera; Control; control methods; Curculionidae; foods; Fundamental and applied biological sciences. Psychology; General agronomy. Plant production; grains; Harvesting. Postharvest. Storage; hexane; high performance liquid chromatography; insect pests; insecticidal properties; insecticide resistance; insecticides; Invertebrates; leaves; Maize weevil; Medically important nuisances and vectors, pests of stored products and materials: population survey and control; methylene chloride; mucosa; nuclear magnetic resonance spectroscopy; pesticide residues; Pests of stored products; Phytopathology. Animal pests. Plant and forest protection; Protozoa. Invertebrates; screening; seeds; Sitophilus zeamais; stored grain; Vegetative propagation. Sowing and planting. Harvesting; Alkaloids; Maize weevil; Bioinsecticide; Acetogenins; Allelochemicals; Sitophilus zeamais; Insecticide; Coleoptera; Insecta; Mucosa; Alkaloid; Pest; Dicotyledones; Angiospermae; Annonaceae; Biopesticide; Pesticides; Stored food product; Biological activity; Biological compound; Arthropoda; Spermatophyta; Curculionidae; Invertebrata; Semiochemicals
• ISSN: 0022-474X; 1879-1212
• DOI: 10.1016/j.jspr.2013.06.001

New control methods are necessary for stored grain pest management programs due to both the widespread problems of insecticide-resistance populations and the increasing concerns of consumers regarding pesticide residues in food products. Thus, this study evaluated the bioactivity of extracts and fractions obtained from different structures (leaves, branches, and seeds) of Annona mucosa (Annonaceae) against Sitophilus zeamais (Coleoptera: Curculionidae), which is a primary insect pest of stored cereals in tropical conditions. In the screening assay, the most promising treatments were extracts prepared from the seeds of Annona mucosa in hexane and dichloromethane (LC90 values of 259.31 and 425.15 mg kg−1, respectively) and, to a lesser extent, an extract prepared from the leaves in hexane (LC90 of 1047.15 mg kg−1). Based on these results and the chromatographic profile of the bioactive crude extracts, the extract prepared from the seeds in hexane was fractionated by liquid–liquid partitioning. The dichloromethane and hydroalcoholic fractions exhibited insecticidal activity against S. zeamais, and no significant difference was observed between these two fractions. The chemical analyses (1H NMR, HPLC, and TLC) showed the presence of alkaloids and acetogenins in the bioactive fractions, which are likely related to the observed bioactivity. Thus, A. mucosa, particularly its seeds, is a promising source of compounds that can be used as a prototype model and/or a biorational insecticide for the control of S. zeamais in stored cereals. •Crude extracts from Annona mucosa were assessed for toxicity to Sitophilus zeamais.•Extracts prepared from seeds and leaves showed promising bioactive effects.•Response levels were dependent on the organic solvent used in the extract preparation.•Bioassay-guided fractionation of seed extract in hexane was performed.•Alkaloids and acetogenins were detected in the bioactive fractions.