Bacterial growth and toxin production in ileostomy effluents

• Published in: Journal of medical microbiology Vol. 28; no. 4; pp. 287 - 290
• Main Authors: Ala aldeen, D. A. A, Barer, M. R
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.04.1989; Society for General Microbiology
• Subjects: Aeromonas - growth & development; Aeromonas - metabolism; Aeromonas sobria; Bacterial Toxins - biosynthesis; Bacteriology; Biological and medical sciences; Colony Count, Microbial; Culture Media; Escherichia coli - growth & development; Escherichia coli - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Ileostomy; Intestinal Secretions - microbiology; Microbiology; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Vibrio cholerae; Vibrio cholerae - growth & development; Vibrio cholerae - metabolism; Enterotoxin; Ileostomy; Biological fluid; Microorganism growth; Production; Gram negative bacteria
• ISSN: 0022-2615; 1473-5644
• DOI: 10.1099/00222615-28-4-287

Department of Clinical Tropical Medicine, The London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT * Correspondence should be sent to: Dr M. R. Barer, Department of Microbiology, University of Newcastle upon Tyne Medical School, Framlington Place, Newcastle upon Tyne NE2 4HH. Received August 22, 1988 Accepted October 10, 1988 Escherichia coli (2), Vibrio cholerae (2) and Aeromonas sobria (1) strains were examined for their ability to grow and produce toxins in samples of ileostomy fluid. Three categories of response were observed: no detectable growth, growth without detectable toxin, and growth with detectable toxin. Clear differences were apparent between samples of ileostomy fluid obtained from different individuals and between samples obtained from the same individual at different times. The patterns of response were unique for each of the five test strains. We propose that the procedure developed forms a basis for investigating the host-parasite relationship in diarrhoeal disease.