Hepatoprotective potential of astaxanthin against 2,3,7,8-tetrachlorodibenzo-p-dioxin in cultured rat hepatocytes

• Published in: Toxicology and industrial health Vol. 30; no. 2; pp. 101 - 112
• Main Authors: Turkez, Hasan, Geyikoglu, Fatime, Yousef, Mokhtar I, Togar, Basak, Gürbüz, Hasan, Celik, Kubra, Akbaba, Giray B, Polat, Zuhal
• Format: Journal Article
• Language: English
• Published: London, England SAGE Publications 01.03.2014; Sage Publications Ltd
• Subjects: Animals; Antioxidants; Antioxidants - pharmacology; Astaxanthin; Biology; Carotenoids; Cell Survival; Cells, Cultured; Culture; Dehydrogenases; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; Deoxyribonucleic acid; Dioxins; DNA Damage - drug effects; Dose-Response Relationship, Drug; Hepatocytes - drug effects; Hepatocytes - metabolism; Hydrocarbons; L-Lactate Dehydrogenase - metabolism; Laboratory animals; Lactate dehydrogenase; Liver; Liver - cytology; Liver - drug effects; Liver - metabolism; Male; Micronucleus Tests; Oxidative stress; Oxidative Stress - drug effects; Polychlorinated Dibenzodioxins - toxicity; Rats; Rats, Sprague-Dawley; Signal transduction; Tetrazolium Salts - metabolism; Thiazoles - metabolism; Toxicity; Toxicology; Viability; Xanthophylls - pharmacology; Turkey; genotoxicity; TCDD; cell viability; cultured rat hepatocytes; oxidative status; astaxanthin
• ISSN: 0748-2337; 1477-0393
• DOI: 10.1177/0748233712452607

The purpose of this study was to evaluate the effect of carotenoid astaxanthin (ASTA) on cultured primary rat hepatocytes treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the cell viability (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT), lactate dehydrogenase (LDH) activity, 8-oxo-2-deoxyguanosine (8-OH-dG), total antioxidant capacity (TAC), and total oxidative stress (TOS) levels, and liver micronucleus rates. ASTA (2.5, 5, and 10 µM) was added to cultures alone or simultaneously with TCDD (5 and 10 µM) for 48 h. The results of MTT and LDH assays showed that both doses of TCDD caused significant decrease in cell viability. Also, TCDD significantly increased TOS and decreased TAC level in rat hepatocytes. On the basis of increasing doses, the dioxin caused significant increase in micronucleated hepatocytes) and 8-OH-dG level as compared to control culture. The presence of ASTA with TCDD minimized its effects on primary hepatocytes cultures and DNA damages.