Comparisons of three automated systems for genomic DNA extraction in a clinical diagnostic laboratory

• Published in: Yonsei medical journal Vol. 51; no. 1; pp. 104 - 110
• Main Authors: Lee, Jong-Han, Park, Yongjung, Choi, Jong Rak, Lee, Eun Kyung, Kim, Hyon-Suk
• Format: Journal Article
• Language: English
• Published: Korea (South) Yonsei University College of Medicine 01.01.2010; 연세대학교의과대학
• Subjects: Automation - methods; DNA - blood; DNA - isolation & purification; Humans; Original; Polymerase Chain Reaction; Reagent Kits, Diagnostic; Reproducibility of Results; 의학일반; nucleic acid extraction; Automated system; DNA; polymerase chain reaction
• ISSN: 0513-5796; 1976-2437
• DOI: 10.3349/ymj.2010.51.1.104

The extraction of nucleic acid is initially a limiting step for successful molecular-based diagnostic workup. This study aims to compare the effectiveness of three automated DNA extraction systems for clinical laboratory use. Venous blood samples from 22 healthy volunteers were analyzed using QIAamp Blood Mini Kit (Qiagen), MagNA Pure LC Nucleic Acid Isolation Kit I (Roche), and Magtration-Magnazorb DNA common kit-200N (PSS). The concentration of extracted DNAs was measured by NanoDrop ND-1000 (PeqLab). Also, extracted DNAs were confirmed by applying in direct agarose gel electrophoresis and were amplified by polymerase chain reaction (PCR) for human beta-globin gene. The corrected concentrations of extracted DNAs were 25.42 +/- 8.82 ng/microL (13.49-52.85 ng/microL) by QIAamp Blood Mini Kit (Qiagen), and 22.65 +/- 14.49 ng/microL (19.18-93.39 ng/microL) by MagNA Pure LC Nucleic Acid Isolation Kit I, and 22.35 +/- 6.47 ng/microL (12.57-35.08 ng/microL) by Magtration-Magnazorb DNA common kit-200N (PSS). No statistically significant difference was noticed among the three commercial kits (p > 0.05). Only the mean value of DNA purity through PSS was slightly lower than others. All the extracted DNAs were successfully identified in direct agarose gel electrophoresis. And all the product of beta-globin gene PCR showed a reproducible pattern of bands. The effectiveness of the three automated extraction systems is of an equivalent level and good enough to produce reasonable results. Each laboratory could select the automated system according to its clinical and laboratory conditions.