Influences and mechanisms of surfactants on pyrene biodegradation based on interactions of surfactant with a Klebsiella oxytoca strain

• Published in: Bioresource technology Vol. 142; pp. 454 - 461
• Main Authors: Zhang, Dong, Zhu, Lizhong, Li, Feng
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.08.2013; Elsevier
• Subjects: Bacteria; Biodegradation; Biodegradation, Environmental; Biological and medical sciences; Biomass; Biotechnology; Cell Membrane Permeability; Cell surface hydrophobicity; Culture Media; Fundamental and applied biological sciences. Psychology; Klebsiella; Klebsiella oxytoca - metabolism; Lipids; Membranes; Pyrene; Pyrenes; Pyrenes - metabolism; Sorption; Surface-Active Agents - metabolism; Surfactant; Surfactants; Sorption; Biodegradation; Pyrene; Surfactant; Cell surface hydrophobicity; Klebsiella oxytoca; Bacteria; Hydrophobicity; Cell surface; Enterobacteriaceae
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2013.05.077

[Display omitted] •Surfactant-induced sorption was the primary contribution in pyrene biodegradation.•Highly positive correlation between Bs∗/B0∗ and Kd,s∗/Kd,0∗ was found.•Alteration of CSH and sorption ability of pyrene depended on surfactants structure. Surfactant-enhanced bioremediation has been proposed as a promising technology for the treatment of organic polluted soils; however its application has been hindered by the controversial influences and mechanisms of surfactants on the biodegradation of hydrophobic organic compounds. To address this problem, effects of five surfactants on the sorption and biodegradation of pyrene by Klebsiella oxytoca PYR-1, as well as their interactions with bacterial cell surface and membrane lipids were investigated. We found that surfactants enhanced or inhibited pyrene biodegradation depending on their effects on the sorption of pyrene onto bacterial cell, which occurred mainly through modifying cell surface hydrophobicity (such as Tween series surfactants) or disrupting bacterial membrane (such as Triton X-100), respectively. A relatively high positive correlation (P<0.0001) was observed between biodegradation promotion (Bs∗/B0∗) and enhancement of sorption coefficients (Kd,s∗/Kd,0∗) for pyrene in the presence of surfactant, indicating that surfactant-induced sorption played the dominant role during pyrene biodegradation.