Characterization of coccidial proteins by two-dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis

Two dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS-PAGE) has been used to produce 'fingerprint' maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few di...

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Bibliographic Details
Published inParasitology Vol. 99; pp. 175 - 187
Main Authors Sutton, C.A, Shirley, M.W, Wisher, M.H
Format Journal Article
LanguageEnglish
Published England 01.10.1989
Subjects
Animals
Antigens, Protozoan - analysis
Autoradiography
Blotting, Western
characterization
Chickens
Eimeria
Eimeria - analysis
Eimeria - immunology
electrophoresis
Electrophoresis, Polyacrylamide Gel
Peptide Mapping
Peptides - analysis
polypeptides
Protozoan Proteins - analysis
strain differences
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Summary:Two dimensional sodium dodecyl sulphate-polyacrylamide gel electrophoresis (2D SDS-PAGE) has been used to produce 'fingerprint' maps of the proteins from each of the 7 species of Eimeria which infect the chicken. All 7 species could be identified from their array of polypeptides but few differences were detected between strains of the same species. Alterations to the polypeptide array associated with the stage of sporulation of the oocysts were observed. Iodination of sporozoites, 2D SDS-PAGE, autoradiography and immunoblotting techniques were combined to identify polypeptides with a surface moiety and those which were antigenic.
ISSN:0031-1820
1469-8161
DOI:10.1017/S0031182000058613